(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH 产品活性：(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (Surfagon，Mwt 1167.34 Da) 是一种促性腺激素释放激素 (GnRH) 激动剂。(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH 能作为醋酸亮丙瑞林 LC-MS 分析的内标。(Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH 在生化分析和生育中有潜在应用。 产品来源： https://www.medchemexpress.cn/des-gly10-d-ala6-pro-nhet9-lhrh.html 研究领域：GPCR/G Protein 作用靶点：GnRH Receptor In Vitro: (Des-Gly10, D-Ala6, Pro-NHEt9)-LHRH as internal standard for leuprorelin acetate (1) Add 10 μL 200 ng/mL (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH solution to 100 μL plasma sample. (2) Add ice-cold acetonitrile to solution in (1) in two batches (500 µL each time). (3) Stir at 2500 rpm for 10 min. (4) Centrifugation at 12500 rpm (4℃) for 10min. (5) Transfer the supernatant into a centrifuge tube, dried under nitrogen gas at 35℃, and finally add 100 µL 0.1% formic acid in water/acetonitrile: 90/10 (v/v) solution. (6) The Thermo Scientific Vanquish UPLC system is connected to the Thermo Scientific Orbitrap Explorer 480 mass spectrometer. Using the Waters Acquity UPLC HSS T3 column (2.1 × 100 mm, 1.8 µm) separation at a temperature of 40℃. (7) The mobile phase A is 0.1% formic acid in water, and the mobile phase B is 0.1% formic acid in acetonitrile solution. The gradient starts at 95% mobile phase A, decreases to 10% over 5 min, and then remains constant for 0.5 min. At 6 min, the column returned to 95% mobile phase A and is rebalanced for 2 min. The flow rate was 0.3 mL/min and the injection volume is 20 µL. (8) The sample is ionized by 3500V electric spray. In positive-ion mode, m/z 605.3275 is leuprolide acetate, and m/z 584.3052 is the internal standard. Data are analyzed by Xcalibur software (version 4.4). In Vivo: (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (2 µg/kg; i.p.; single dose) significantly increases anxiety behavior in adult male rats with and without gonadectomy. (Des-Gly10,D-Ala6,Pro-NHEt9)-LHRH (50 µg; single dose) improves the fertility of cows after fertilization and prevents embryo death when combined with Ainil (30 µL/kg). 相关产品：Degarelix | Goserelin acetate | Cetrorelix Acetate | BAY-899 | Relugolix | Alarelin Acetate | Fertirelin | Elagolix sodium | Kisspeptin-54(human) | Lecirelin | BAY-298 | Abarelix Acetate | Histrelin acetate | Luteinizing Hormone Releasing Hormone (LH-RH), salmon | Prolactin Releasing Peptide (1-31), human acetate | sGnRH-A acetate | Sufugolix | Antide | Acyline TFA | GnRH Associated Peptide (GAP) (1-13), human | NBI-42902 | Phoenixin-14 | (D-His2,D-Trp6)-LHRH | (D-Trp6)-LHRH free acid 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。