Coumarin 6 产品活性：Coumarin 6 是一种荧光染料，可作为荧光探针用于微粒给药系统中，对活性分子给药系统进行体内跟踪、细胞摄取和转运机制研究 (λexc=450 nm，λem=505 nm)。 产品来源： https://www.medchemexpress.cn/coumarin-6.html 研究领域：Others 作用靶点：Fluorescent Dye 来源：植物 | 菊科 | 滨海豚草 | 植物 | 伞形科 结构分类：生物碱 | 吲哚类生物碱 | 结构分类 In Vitro: Coumarin 6 labeled HPMC nanoparticles (1) Dissolve Coumarin 6 in DMF to prepare a 5 mg/mL organic phase solution. (2) Dissolve hydroxypropyl methyl cellulose (HPMC) in HBSS to prepare a 50 μg/mL aqueous solution. (3) Add the organic solution to the aqueous solution using a MasterFlex L/S pump (volume ratio of organic solution to aqueous solution is 1:100) with an injection rate of 36 mL/min. (4) Prepare 70 nm nanoparticles by stirring at room temperature for 5 min at a stirring speed of 9 mL/min. (5) Add 2 μg/mL nanoparticles to the MDCKII cell culture dish and incubate in the dark for 5 min, 15 min, 30 min and 60 min. (6) Add cold PBS buffer to stop the uptake of nanoparticles by MDCKII cells and clean with HBSS. (7) Use a fluorescence microscope to observe the uptake of nanoparticles by MDCKII cells. 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。