耗材描述
杀稻瘟菌素 S 无菌溶液
MCE Blasticidin S, Sterile 来源于灰色链霉菌(Streptomyces griseochromogenes),是一种核苷类抗生素,主要通过干扰核糖体中肽键的形成而特异性抑制原核细胞或真核细胞的蛋白合成。
产品来源: https://www.medchemexpress.cn/inhibitor-kit/blasticidin-s-sterile.html
Description & Advantages: Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It can be used as a selection agent in both mammalian cells and bacterial cells.
Resistance to Blasticidin S is conferred by bsr and BSD. Therefore, Blasticidin S is a popular and effective antibiotic for selecting vectors bearing the bsr or BSD gene in mammalian cell lines. The recommended working concentration ranges from 1 to 20 μg/mL depending on the cell line and 50–100 μg/mL for bacterial selection. For bacterial selection purposes, the salt content of the LB medium must remain low (
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