耗材描述
反转录试剂盒 II (预混 gDNA 消化酶)
RT Master Mix for qPCR II (gDNA digester plus) 反转录即用型预混液,采用升级款反转录酶,反应更高效、快速,适用于高 GC 含量、复杂二级结构、少量 RNA 模板等。试剂盒配有 gDNA digester 可去除 RNA 模板中的基因组 DNA 污染。
产品来源: https://www.medchemexpress.cn/inhibitor-kit/rt-master-mix-for-qpcr-ii-gdna-digester-plus.html
Description & Advantages: MCE RT Master Mix is a convenient, ready-to-use formulation for reverse transcription. This kit contains 5x gDNA digester Mix which can eliminate gDNA contaminations in RNA samples. 4× Super RT Mix contains all the reagents necessary for first-strand cDNA synthesis. In addition, the increased heat resistance of the advanced reverse transcriptase allows reverse transcription to be performed at a higher temperature, increasing the efficiency and specificity of cDNA synthesis.
Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR and qPCR. MCE SYBR Green qPCR Master Mix (HY-K0501) is highly recommended for detection of the expression levels of interested genes.
Features:
1. Synthesize cDNA rapidly and specifically.
2. Super-fast gDNA removal in 2 minutes.
3. Optimized buffer enhances sensitive and reliable cDNA synthesis.
4. Super-efficient reaction even with low template amounts (total RNA: 50 pg -5 μg in a 20 μL reaction).
5. The kit provides both Oligo dT Primer and Random Primer.
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