耗材描述
Vari Fluor 680-Streptavidin
产品活性:Vari Fluor 680-Streptavidin 是一种 Vari Fluor-链霉亲和素类染料,由 Vari Fluor 系列荧光探针标记链霉亲和素而成。链霉亲和素(Streptavidin)是一种具有高度亲和力的四聚体蛋白,每个四聚体由四个相同的链霉亲和素亚基组成。链霉亲和素能够特异性结合生物素,形成可逆的非共价作用。链霉亲和素可实现生物素标记物的快速和高效检测,常用于免疫荧光 (IF)、酶联免疫吸附实验(ELISA)、免疫组化染色 (IFH)、原位杂交等实验。Ex/Em=680 nm/701
产品来源: https://www.medchemexpress.cn/vari-fluor-680-streptavidin.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: General Protocol
1.Protein treatment
Before use, centrifuge at 5000× g for 3 min, only the supernatant is used for experiments to eliminate protein aggregates and reduce non-specific background staining.
2.Labeling
Dilute Vari Fluor-Streptavidin at 1:500-1:1000, which can be adjusted according to the specific conditions of the protein or antibody.
Storage
-20°C,
Protect from light
Precautions
1. The actual content of the dye is small, dissolve it directly in the tube after receiving it for experiments.
2. Before use, please centrifuge the product to the bottom of the tube instantaneously before subsequent experiments.
3. This product is intended for scientific research only by professionals and is not to be used for clinical diagnosis or treatment, food or medicine.
4. For your safety and health, please wear lab coat and disposable gloves.
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