Fluo-3FF AM 产品活性：Fluo-3FF AM 是一种低亲和力 (Kd = 42 μM) 荧光 Ca2+ 敏感指示剂 (激发和发射极值为 462 nm/526 nm)。Fluo-3FF AM 对 Mg2+ 不敏感并且具有相对较高的光稳定性。Fluo-3FF AM 是 Fluo-3FF AM 的类似物，Fluo-3FF AM 本质上是无荧光的，但在进入细胞并与钙结合时表现出强烈的荧光增强。 产品来源： https://www.medchemexpress.cn/fluo-3ff-am.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: 1. Preparation of Fluo-3FF AM working solution 1.1 Preparation of storage solution Fluo-3FF AM was diluted with a 0.025% (w/v) solution of Pluronic F-127/DMSO to make a 1 mM stock solution. Note: Fluo-3FF AM stock solution is recommended to be stored in the dark at -20°C or -80°C after aliquoting. 1.2 Preparation of working solution Prepare a 5 μM Fluo-3FF AM working solution with equilibration buffer. Note: Please adjust the concentration of Fluo-3FF AM working solution according to the actual situation, and prepare it immediately after use. 2. Cell staining 2.1 Adherent cells were cultured on sterile cover slips. 2.2 Remove the cover slip from the medium and remove excess medium by suction. 2.3 At 37°C, 100 μL of dye working solution was added and gently shaken to completely cover the cells and incubated for 60 minutes. 2.4 Aspirated the dye working solution, then washed in PSS at 4℃ for 60 minutes. 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。