EDTA-AM 产品活性：EDTA-AM (ethylenediaminetetraacetic acid, acetoxymethyl ester) 是金属离子螯合剂 EDTA 的膜渗透形式。活细胞通过与 EDTA-AM 孵育而负载 EDTA-AM ，一旦内化胞浆酯酶分解 AM，释放活性配体 EDTA，从而分离细胞内的金属离子。EDTA-AM 诱导有丝分裂进程停滞和染色体解聚。 产品来源： https://www.medchemexpress.cn/edta-am.html 研究领域：Others 作用靶点：Biochemical Assay Reagents In Vitro: EDTA-AM treatment of mitotic cells： 1. Mitotic cells were collected and seeded onto a 12-well culture plate with poly lysine-coated coverslips and DMEM supplemented with 10% FBS. 2. EDTA-AM was dissolved in 100 mM DMSO, EDTA-AM was added to the culture medium at a final concentration of 20 mM with 0.02% Pluronic F-127 (Invitrogen), and the cells were further incubated at 37C for 100 min. 3.After incubation, the numbers of mitotic cells and interphase cells were counted. The cells on the coverslips were fixed, stained with DAPI, and mounted with para-phenylene diamine (PPDI) solution (20 mM HEPES, pH 7.4, 1 mM MgCl2, 100 mM KCl, 78% glycerol, 1 mg/mL PPDI). 相关产品：PEG300 | Tween 80 | Corn oil | SBE-β-CD | Hexadimethrine bromide | Solutol HS-15 | Methyl-β-cyclodextrin | HP-β-CD | Cremophor EL | Sodium carboxymethyl cellulose (Viscosity:800-1200 mPa.s) | DL-dithiothreitol | IPTG | Phosal 50 PG | PLGA (50:50) | EGTA | Ac4ManNAz (80% α isomer) | Methyl cellulose | Bovine Serum Albumin | DTNB | FITC-Dextran (MW 70000) | LysoTracker Red | Biotin-azide | 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride | Trifluoromethanesulfonic acid silver | Ethylenediaminetetraacetic acid | NAI-N3 | Gelucire 44/14 | HEPES | Formamide 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。