DAPI dilactate 产品活性：DAPI (dilactate) 是一种蓝色荧光染料，优先结合 dsDNA 并与小沟 AT 簇结合。DAPI (dilactate) 与 dsDNA 结合使荧光增强约 20 倍。DAPI (dilactate) 可用于识别细胞周期，可特异性染色细胞核，但不染色细胞质。DAPI (dilactate) 与DAPI (dihydrochloride) 形式相比更易溶于水。 产品来源： https://www.medchemexpress.cn/dapi-dilactate.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: General protocol 1. Preparation of DAPI dilactate working solution 1.1 Preparation of stock solution Dissolve 5 mg DAPI dilactate in 1 mL ddH2O to obtain 5 mg /mL stock solution (DAPI, dilactate 10.9 mM). Note: It is recommended to store the stock solution at -20°C or -80°C in the dark and avoid repeated freezing and thawing. 1.2 Preparation of DAPI dilactate working solutionThe stock solution was diluted 1:5000 in PBS to 1 μg/mL. Store the working solution at 4 °C. Note: Please adjust the concentration of DAPI dilactate working solution as needed 2. Cell staining 2.1 Suspension cells (96-well plate) a. Centrifuge at 1000 g for 3-5 min at 4°C and discard the supernatant. Wash with PBS twice, 5 minutes each time. The cell density is 1×106/mL. b. Add 1 mL of working solution and incubate at room temperature for 3-10 minutes.c. Centrifuge at 400 g for 3-4 minutes at 4°C and discard the supernatant.d. Wash 2 times with PBS, 5 min each.e. Resuspend cells in serum-free cell culture medium or PBS. Fluorescence microscopy or flow cytometry observation. 2.2 Adherent cells a. Adherent cells were cultured on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, shake gently to completely cover the cells, and incubate at room temperature for 3-10 minutes.d. Wash 2 times with medium, 5 min each. Fluorescence microscope or flow cytometry observation. Precautions For long-term storage the stock solution can be aliquoted and stored at ≤–20°C. For shortterm storage the solution can be kept at 2–6°C, protected from light. When handled properly, DAPI solutions are stable for at least six months. 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。