MitoTracker Orange CMTMRos 产品活性：MitoTracker Orange CMTMRos 是一种荧光染料，利用线粒体膜电位标记活细胞内的线粒体 (Ex/Em: 551/576 nm)。 产品来源： https://www.medchemexpress.cn/mitotracker-orange-cmtmros.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: For live cells, follow Steps 1–7. For fixed cells, follow Steps 8–13. 1. Prepare a labeling solution at 25–500 nM in the desired cell culture medium, and warm it to the cell culture temperature. 2. Aspirate the culture medium from cells grown on coverslips. 3. Immerse the cells in the labeling medium for 15–45 min at 37?C. 4. Aspirate the labeling medium, and rinse the cells three times with culture medium. 5. Mount the cells. 6. Check the cells using a fluorescence microscope. If the fluorescence staining is too low, try one of the following options: i. Incubate for an additional 30 min in the normal culture medium to allow the thiol conjugation to proceed. ii. Try a higher initial concentration of the labeling solution. 7. When the fluorescence is sufficient, image the cells. 8. Carry out Steps 1–4, but use a labeling solution at a working concentration between 100 and 1000 nM. 9. Rinse the cells three times with PBS+. 10. Fix the cells for 10 min in 3.7% formaldehyde in PBS (pH 7.4). 11. Aspirate and rinse the cells three times for 5 min each in PBS. 12. Mount the coverslips. 13. Image the cells. 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。