耗材描述
FBBBE
产品活性:FBBBE 可用于检测细胞产生的H2O2。FBBBE 可被细胞内 H2O2 触发并转化为荧光素,导致细胞内荧光增加 (Ex=4480 nm, Em=512 nm)。
产品来源: https://www.medchemexpress.cn/fbbbe.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. The RAW 264.7 cells are seeded at 250,000 in glass bottom 35 mm dishes and post-adhering (5 h).
2. Some dishes are treated with 1 μg/mL of LPS for 24 h. Other dishes are treated the next day after plating with either 100 μM H2O2 for 1 h or PBS for 1 h.
3. Cells are washed twice with PBS and fixed in ice-cold 95% ethanol for 15 min.
3. Cells are twice rinsed with PBS and incubated with either 50 μM probe (FBBBE) for 1 h at room temperature.
4. Cells are washed twice with PBS and coverslips were mounted over cells using Aqua Mount.
5. Confocal images are obtained at 80× magnification on an Olympus FV1000 confocal laser scanning microscope.
6. Images were processed using ImageJ software.
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