o-Pah 产品活性：o-Pah 是一种带有 -NH2 和 -OH 取代的 meso-Ph 基团的 BODIPY 衍生物。o-Pah 在 Cu2+ 存在下，表现为金属诱导的 J 聚集和对 Hg2+ 特定荧光增强 (Ex/Em=483/(495-600) nM)。 产品来源： https://www.medchemexpress.cn/o-pah.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: o-Pah (20 μM) shows changed colour upon addition of 100 μM different metal ions and the further addition of 20 μM of Cu2+ in DMSO/HEPES buffer solution. Procedures of metal ion sensing Stock solutions of the metal ions (100 mM and 10 mM) were prepared in deionised water. 1. A stock solution of o-Pah (0.5 mM) is prepared in DMSO. 2. The solution of o-Pah is then diluted to 5 μM with HEPES buffer solution (50 mM KNO3, 50 mM HEPRS, pH 7.2). 3. During titration experiments, 2 mL solutions of o-Pah (5 μM, 0.5 μM) are placed in a 1 cm quartz optical cell and Cu2+, Hg2+ stock solutions are added gradually with a micropipette. 4. In selectivity experiments, the test samples are prepared by adding appropriate amounts of the metal ion stock solution into the 2 mL solution of o-Pah (5 μM). 6. During fluorescence measurements, the excitation wavelength is 483 nm and emission spectra are collected between 495-600 nm. 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。