4-Methylumbelliferyl phosphate disodium 产品活性：4-Methylumbelliferyl phosphate (4-MUP) disodium 是一种阴离子有机磷酸酯，是一种酸性和碱性磷酸酶 (phosphatase) 的荧光底物。4-Methylumbelliferyl phosphate disodium 也是一种神经毒剂模拟剂。 产品来源： https://www.medchemexpress.cn/4-methylumbelliferyl-phosphate-disodium.html 研究领域：Metabolic Enzyme/Protease 作用靶点：Phosphatase In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Serum acid phosphatase assay: 1. Preparation a 100 μL solution containing the following agents: 5.0 μL of serum enzyme, 50 μL of 4-Methylumbelliferyl phosphate (5.0 mM), and 10 μL of 1.0 M buffer at pH 6.0. 2. Add the following agents (with the final concentration) 20 mM Sodium Tartrate (HY-128476), 15 mM Sodium Fluoride (HY-B1766), 3000 mM 2-Mercaptoethanol, 4 mM 1, 10-Phenanthroline (HY-W004544), 6 mM 8-Quinolinol (HY-B1005), 6 mM 8-Hydroxy-5quinolinesulfonic acid, 45 mM EDTA (HY-Y0682) and 6 mM EGTA (HY-D0861). 3. Incubate reaction mixtures for 15 min at 37℃ and terminated by the addition of 2.9 mL of 0.1 M ammonium hydroxide/glycine buffer, pH 10.5. 4. Mix samples thoroughly and determine fluorescence by a fluorometer. 相关产品：Cyclosporin A | Tacrolimus | SHP099 | Calyculin A | β-Glycerophosphate disodium salt pentahydrate | Okadaic acid | Salubrinal | Sodium orthovanadate | Ebselen | Batoprotafib | L-Ascorbic acid 2-phosphate trisodium | Suramin sodium salt | Osunprotafib | RMC-4550 | TPI-1 | GSK 2830371 | Sodium metatungstate | DT-061 | SF1670 | Vociprotafib | NSC 663284 | Idoxuridine | D-erythro-Sphingosine | MSI-1436 lactate | BCI | LB-100 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。