耗材描述
Rhod-2 AM
产品活性:Rhod-2 是一种高亲和可见光激发波长的 Ca2+ 荧光探针,Rhod-2 AM 是 Rhod-2 的乙酰甲酯衍生物,具有细胞膜通透性,简单培养即可轻松进入细胞。一旦进入细胞,被其乳糖酶剪切产生无膜渗透性的 Rhod-2,留在细胞内执行相应的生理功能。最大激发/发射波长: 549/578 nm。
产品来源: https://www.medchemexpress.cn/Rhod-2_AM.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: 1.Preparation of Rhod-2 AM working solution
1.1Preparation of the stock solution
Dissolve Rhod-2 AM in DMSO to obtain 5 mM of stock solution.
1.2Preparation of Rhod-2 AM working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of working solution.
Note: Please adjust the concentration of Rhod-2 AM working solution according to the actual situation.
2.Cell staining (6-well plate)
2.1Suspension cells
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d.Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.
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