Acridine Orange base 产品活性：Acridine Orange base 是一种细胞通透性荧光染料，能将生物体 (细菌，寄生虫，病毒等) 染成亮橙色，而当在适当条件下 (pH=3.5, Ex=460 nm) 可将人类细胞区别性地染成绿色，以便用荧光显微镜进行检测。Acridine Orange base 与 dsDNA 结合时发出绿色荧光 (Ex=488, Em=520-524)，与 ssDNA (Ex=457, Em= 630-644) 或 ssRNA 结合时发出红色荧光 (Ex=457, Em=630-644)，也可用于细胞周期测定。 产品来源： https://www.medchemexpress.cn/acridine-orange-base.html 研究领域：Anti-infection 作用靶点：Parasite In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Differential staining of DNA and RNA of unfixed cells: (1) Set up the flow cytometer with excitation at 488 nm, using emission filters and a dichroic mirror that discriminate green fluorescence (measured at 515-545 nm) and red luminescence (measured preferably above 640 or 650 nm). (2) Transfer a 0.2-mL aliquot of the original cell suspension to a small glass or plastic tube (e.g., 2- or 5-mL volume). Chill on ice. (3) Gently add 0.4 mL ice-cold cell permeabilizing solution. Wait 15 s, keeping cells on ice. (4) Gently add 1.2 mL ice-cold Acridine Orange base staining solution. Keep cells on ice in the dark. (5) Measure and record cell fluorescence in the flow cytometer during the 2 to 10 min after addition of Acridine Orange base staining solution. 2. Differential staining of fixed cells: (1a) For cells in suspension culture or hematologic samples: Rinse cells once with ice-cold PBS and suspend in ice-cold PBS at ∼106 cells/mL. (1b) For cells attached to tissue culture plates: Collect cells from flasks or petri plates by trypsinization, pool the trypsinized cells with cells floating in the medium (mostly detached mitotic and dead cells), and rinse once with medium containing serum to inactivate the trypsin. Suspend cells in ice-cold PBS at ∼106 cells/mL. (1c) For cells isolated from solid tumors: Rinse cells free of any enzyme used for cell dissociation and suspend in ice-cold PBS at ∼106 cells/mL. (2) With a Pasteur pipet transfer 1 mL cell suspension to a 15-mL conical glass tube containing 10 mL ice-cold 70% ethanol. Fix cells ≥2 h on ice. (3) Centrifuge tubes 5 min at 300 × g, 4℃. Remove all ethanol, rinse cells once with ice-cold PBS, and suspend in ice-cold PBS at a density of 6 cells/mL. (4) Withdraw 0.2 mL cell suspension (≤ 2 × 105 cells) and transfer to a small tube (e.g., 2 or 5 mL volume). Chill on ice. (5) Add 0.4 mL ice-cold permeabilizing solution. Wait 15 s, keeping cells on ice. (6) Add 1.2 mL ice-cold Acridine Orange base staining solution. Keep cells on ice. (7) Measure and record cell fluorescence in the flow cytometer during the 2 to 10 min after addition of Acridine Orange base staining solution. 相关产品：Chloroquine | Puromycin dihydrochloride | Hygromycin B | Doxycycline hyclate | Acetaminophen | Anisomycin | Metronidazole | Kaempferol | Amphotericin B | Hydroxychloroquine | Monensin sodium | Dihydroartemisinin | Niclosamide | Berberine chloride | Artesunate | Acridine Orange hydrochloride | Ivermectin | Eflornithine hydrochloride hydrate | Azithromycin | TPE-MI | Halofuginone | Ibuprofen | Artemisinin | 5'-Methylthioadenosine | RRx-001 | Cepharanthine | Betulinic acid 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。