6-CFDA 产品活性：6-CFDA 是一种常见的脂肪族荧光素系列物。CFDA 可在细胞内自由扩散，并被细胞内非特异性脂肪酶水解生成羧基荧光素 (CF)。与荧光素染料相比，含有 CF 的部分带有额外的负电荷，使它能够更好地保留在细胞中。 产品来源： https://www.medchemexpress.cn/6-CFDA.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: Preparation of 6-CFDA working solution 1.Preparation of the stock solution Dissolve 1mg 6-CFDA in 0.2172 mL DMSO to obtain 10 mM of 6-CFDA. Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles. 2.Preparation of 6-CFDA working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of 6-CFDA working solution. Note: Please adjust the concentration of 6-CFDA working solution according to the actual situation. Cell staining 1.Cell preparation:For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2.Add 1 mL of 6-CFDA working solution, and then incubate at room temperature for 30 minutes. 3.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. 4.Wash twice with PBS, 5 minutes each time. 5.Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer. Precautions 1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles. 2. Please adjust the concentration of 6-CFDA working solution according to the actual situation. 3. This product is for R&D use only, not for drug, household, or other uses. 4. For your safety and health, please wear a lab coat and disposable gloves to operate. 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。