耗材描述
Merocyanin 540
产品活性:Merocyanin 540 是一种荧光膜探针,可选择性地染色各种电兴奋细胞的膜,但不染色非兴奋细胞的膜 (Ex/Em: 540/580 nm)。
产品来源: https://www.medchemexpress.cn/merocyanin-540.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Wash monolayer cultured cells with isotonic saline with or without 1-5 mM CaCl2 or iso-osmotic sucrose (0.25 M) solutions buffered with 5 mM Tris-Cl (pH 7.4).
2. Expose cells to Merocyanin 540 (1.6 x 10-5 M) in the appropriate buffer for 10 min at room temperature under ambient lighting conditions.
3. Wash extensively and examine by fluorescence microscopy.
4. For protozoa, place 0.05 mL aliquots of washed cultures containing 100-200 organisms on a microscope slide.
5. Add Merocyanin 540 to a final concentration of 1.0-10 μg/mL.
6. Examine cells by fluorescence microscopy.
7. Incubate single cell suspensions or tissue slices with Merocyanin 540 (1.75-5.25 x 10-5 M) and examine by fluorescence microscopy as described for attached cells.
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