Cy3 methyltetrazine 产品活性：Cy3 methyltetrazine (TZ-Cy3) 是一种具有甲基四嗪结构单元的点击化学试剂，其对环辛烯具有高度反应性。Cy3 methyltetrazine 也是一种四嗪修饰的荧光探针，可用于分析溶液和活细胞中的蛋白质磷酸化。 产品来源： https://www.medchemexpress.cn/cy3-methyltetrazine.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Confocal imaging of phosphorylation in cells: 1. Culture H1299 cells in a coverglass-bottom imaging dish for 24 h (take H1299 cells as an example). 2. Incubate H1299 cells with different concentrations of ATP-NB (a ATP analogue functionalized by norbornene, which can penetrate cells and efficiently phosphorylate proteins in living cells) for 1 h. 3. Add Cy3 methyltetrazine to label the phosphorylated protein with fluorescence. 4. Incubate with fresh medium for 1 h. 5. Wash the cells twice with PBS to release the free Cy3 methyltetrazine probe. 6.Incubate the cells with 4% paraformaldehyde for 15 min and with 0.1% Triton X-100 solution at room temperature for 10 min. 7. Wash cells 4 times with PBS. 8. Use a confocal microscopy imaging system to obtain the high-resolution images of cells (Ex=488 nm; Em=561 nm). 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。