耗材描述
DDAO-C6
产品活性:DDAO-C6 是一种酯环酮衍生物,是用于检测人血清白蛋白 (HSA) 的高度特异性荧光探针。DDAO-C6 也是一种酶激活的近红外荧光探针,用于目测肠道微生物内源性脂肪酶 (Ex/Em=600/658 nm)。
产品来源: https://www.medchemexpress.cn/ddao-c6.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: DDAO-C6 (10 μM; 30 min; 37 ℃) shows a linear relationship between the fluorescence intensities and lipase concentrations (0-50 μg/mL), and products a red fluorescence signal in the presence of active lipase.
DDAO-C6 hydrolysates have fluorescence properties, with excitation wavelength=550-600 nm, emission spectrum=630-700 nm.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Intestinal Fungi:
1. Disperse fresh intestinal secretions in sterile water and coate on a potato agar plate, containing penicillin (100 U/mL)/streptomycin (0.1 mg/mL) to inhibit intestinal bacteria.
2. Culture at 32 °C for about 5 days until the development of obvious fungal colonies.
3. Spray DDAO-C6 (100 μM) on colonies and incubate at 32 °C for 2 h.
4. Image plate samples on a Amersham Typhoon RGB (λex = 635 nm, λem = 670 ± 15 nm).
In Vivo: DDAO-C6 can be used to guide the rapid identification and cultivation of lipase active fungal strains from intestinal microbes in human feces, which is a potential technique for the biological investigation of intestinal microbes.
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