MKT-077 产品活性：MKT-077 (FJ-776) 是一种高水溶性线粒体染料，具有显著的抗肿瘤活性。MKT-077 的细胞毒性低，对广谱人癌细胞系 (结肠癌、乳腺癌、胰腺癌) 有抑制活性。MKT-077 可抑制裸鼠异植肿瘤模型的肿瘤生长。其荧光波长：Ex/Em=488/543 nm。 产品来源： https://www.medchemexpress.cn/MKT-077.html 研究领域：Others | Cell Cycle/DNA Damage | Metabolic Enzyme/Protease 作用靶点：Fluorescent Dye | HSP In Vitro: Preparation of MKT-077 solution 1.1 Preparation of the stock solution Dissolve 1 mg MKT-077 in 0.2315 mL DMSO to obtain 10 mM of MKT-077 . Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of MKT-077 working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of MKT-077 working solution. Note: Please adjust the concentration of MKT-077 working solution according to the actual situation. Cell staining 2.1 Cell preparation.For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. 2.2 Add 1 mL of MKT-077 working solution, and then incubate at room temperature for 30 minutes. 2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. 2.4 Wash twice with PBS, 5 minutes each time. 2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer. In Vivo: Systemic administration of MKT-077 significantly delays the growth of TT xenografts in mice throughout the treatment. At the end of MKT-077 treatment, it is found that tumor weights are about two-times less in MKT-077-treated group than in control group. MKT-077 treatment also results in weight loss and general toxicity in animals. Results show that the succinate-induced, ADP-stimulated respiratory rate in mitochondria isolated from the liver of rats treated with a bolus i.v. injection of 15 mg MKT-077 1kg body weight each day for 5 days is significantly lower than that of untreated controls. 相关产品：Tamoxifen | MitoSOX Red | BODIPY 493/503 | Palmitic acid | D-Luciferin potassium | Polyinosinic-polycytidylic acid | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Tanespimycin | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | VER-155008 | Indocyanine green | PKH 26 | Geldanamycin 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。