Homoharringtonine 产品活性：Homoharringtonine (Omacetaxine mepesuccinate;HHT) 是具有抗肿瘤特性的细胞毒性生物碱，其通过抑制翻译延长来起作用。 产品来源： https://www.medchemexpress.cn/Homoharringtonine.html 研究领域：JAK/STAT Signaling | Stem Cell/Wnt 作用靶点：STAT 来源：植物 | 三尖杉科 | 三尖杉 结构分类：生物碱 | 吡咯类生物碱 | 结构分类 In Vitro: Homoharringtonine inhibits IL-6-induced STAT3 phosphorylation in a dose- and time-dependent manner. Homoharringtonine (HHT) inhibits cells growth, cell viability and colony formation, as well as induced cell apoptosis through mitochondria pathway. The cytotoxicity of Homoharringtonine on human NSCLC cell lines is investigated, A549 (wild type EGFR) and NCI-H1975 (H1975, mutant EGFR with L858R and T790M), Gefitinib is used as a control. By MTT assay, Homoharringtonine has moderate cytotoxicity to A549 with an IC50 of 3.7 μM and H1975 cells are more sensitive to Homoharringtonine with an IC50 of 0.7 μM. Homoharringtonine inhibits the cell proliferation and growth of A549 cells and H1975 cells in a time- and dose-dependent manner through MTT assay. By trypan blue exclusion assay, Homoharringtonine rapidly reduces viable A549 and H1975 cells in a dose- and time-dependent manner. Homoharringtonine significantly inhibits the clonogenic ability of A549 and H1975 cells. In Vivo: Homoharringtonine (10 mg/kg) efficiently represses tumor growth compared to vehicle control or Gefitinib (P<0.05). Additionally, Homoharringtonine (HHT) treatment does not reduce the mice body weight, which suggests that Homoharringtonine has no apparent side effect. All the mice are euthanized, the tumors are isolated and imaged and the tumor sample cells are harvested to extract protein for determination if Homoharringtonine inhibits STAT3 phosphorylation via western blot. The level of STAT3 phosphorylation and MCL1 from Homoharringtonine treatment group is significantly decreased compared to vehicle control or Gefitinib treatment. Meanwhile, consistant with the results in the above, AKT1/2/3 and ERK1/2 phosphorylation is not inhibited with Homoharringtonine treatment. To further examine the STAT3 phosphorylation in the xenograft tumor samples with different treatments, the tumor samples are frozen and cutted into 10 μm sections for fluorescent immunohistochemistry. Homoharringtonine treatment inhibits STAT3 phosphorylation compared to vehicle control or Gefitinib treatment. 相关产品：Natural Product Library Plus | Drug Repurposing Compound Library Plus | FDA-Approved Drug Library Plus | FDA-Approved Drug Library Mini | Bioactive Compound Library Plus | Immunology/Inflammation Compound Library | JAK/STAT Compound Library | Kinase Inhibitor Library | Stem Cell Signaling Compound Library | Natural Product Library | FDA-Approved Drug Library | Anti-Cancer Compound Library | Small Molecule Immuno-Oncology Compound Library | Anti-Aging Compound Library | Drug Repurposing Compound Library | Differentiation Inducing Compound Library | NMPA-Approved Drug Library | Traditional Chinese Medicine Active Compound Library | FDA Approved & Pharmacopeial Drug Library | Alkaloids Library | Anti-Breast Cancer Compound Library | Anti-Lung Cancer Compound Library | Drug-Induced Liver Injury (DILI) Compound Library | Anti-Pancreatic Cancer Compound Library | Anti-Blood Cancer Compound Library | Anti-Obesity Compound Library | Transcription Factor-Targeted Library | Anti-Liver Cancer Compound Library | Rare Diseases Drug Library | Anti-Cancer Natural Product Library | Anti-inflammatory Traditional Chinese Medicine Active Compound Library | Plant-Sourced Natural Product Library | FDA-Approved Anticancer Drug Library | Anti-Prostate Cancer Compound Library | Cancer Stem Cells Compound Library | Off-patent Drug Library | Highly Selective Inhibitors Library | FDA-Approved Traditional Chinese Medicine Active Compound Library | Anti-Hematopathy Compound Library | Bioactive Compound Library Max | Stattic | Fludarabine | AG490 | Cilengitide | Niclosamide | AS1517499 | C188-9 | Artesunate | TPCA-1 | Colivelin TFA | Napabucasin | WP1066 | NSC 74859 | STAT5-IN-1 | Butyzamide | Cryptotanshinone | Astaxanthin | Scutellarin | Pimozide | RO8191 | AC-4-130 | SD-36 | Glycodeoxycholic Acid | Saikosaponin D | Cucurbitacin I | Diosgenin | 2-NP | BP-1-102 | Nifuroxazide 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。