耗材描述
Flutax-2 (5/6-mixture)
产品活性:Flutax-2 (5/6-mixture) 是紫杉醇的活性荧光衍生物。Flutax-2 (5/6-mixture) 与聚合的 α,β 微管蛋白二聚体结合。激发/发射波长:496/524 nm。紫杉醇是一种由红豆杉属植物产生的二萜次生代谢物,可用于多种癌症的研究。
产品来源: https://www.medchemexpress.cn/flutax-2.html
研究领域:Others | Cell Cycle/DNA Damage | Cytoskeleton
作用靶点:Fluorescent Dye | Microtubule/Tubulin
In Vitro: Preparation of Flutax-2 working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of Flutax-2 in 1 mL of DMSO.
Note: It is recommended to store the stock solution at -20℃or -80℃away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Flutax-2 working solution
Dilute the stock solution in sacetate buffer to obtain 100 nM-1 μM of Flutax-2 working solution.
Note: Please adjust the concentration of Flutax-2 working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Flutax-2 working solution, and then incubate at room temperature for 10-30 minutes.
2.3 Centrifuge at 400 g at 4℃for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Storage
-20℃, 1 year
Protect from light
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