FITC-Dextran (MW 4000) 产品活性：FITC-Dextran (MW 4000) 是一种异硫氰酸荧光素 (FITC) 葡聚糖荧光探针 (Ex=495 nm; Em=525 nm)。FITC-Dextran (MW 4000) 可作为一种标记物来揭示热休克引起的细胞损伤，并研究细胞凋亡的早期和晚期阶段。FITC-Dextran (MW 4000) 还可用于细胞渗透性的研究，如血脑屏障通透性以及血脑屏障破坏程度的测定。 产品来源： https://www.medchemexpress.cn/fitc-dextran-mw-4000.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of cells: For use with apoptotic HeLa cells and human peripheral blood mononuclear cells (PBMC) (viable HeLa and PBMC can not be stained by FITC-Dextran). 1. Incubate cells at 43.5°C for 1 hour and at 37°C for 8 hours to induce apoptosis. 2. Suspend the cells in 100 μL of medium, and mix in Q-prep tubes with 10 μL of propidium iodide (PI), 10 μL of FITC-Dextran (MW 4000) (the final concentration of PI and FITC-Dextran (MW 4000) is 7.5 μM and 1.13 μM, respectively). 3. Incubate cells for 25 min at room temperature in the dark. 4. Take the labeled cells with 3 mL of medium and centrifuge for 10 min at 500 g. 5. Take centrifuged cells with 1 mL of medium and use flow cytometry or fluorescence microscopy analyze (PI: Ex=500 nm, Em=600 nm; FITC-Dextran (MW 4000): Ex=495 nm, Em=525 nm). Paracellular permeability measurement 1. Add FITC-dextran (0.1 mg/mL) to the basal media in the transwell chamber. 2. Collect media from the transwell insert after 15 min. 3. Measure the fluorescence signal (Ex=485 nm, Em=538 nm). 4. Calculate FITC-dextran concentration based on fluorescence intensity. 5. Calculate permeability. In Vivo: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). For intestinal barrier function assay 1. Fast mice for 4 h. 2. Orally gavage mice with FITC-Dextran MW 4000 (0.6 mg/g). 3. Measure fluorescence intensity of plasma in 4 h (excitation nm/emission 520 nm). 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | Dihydroethidium | CFDA-SE | 2-NBDG | FITC-Dextran (MW 10000) | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE | TMRM Perchlorate 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。