D-Luciferin 产品活性：D-Luciferin 是荧光素酶 (Luc) 的天然底物，对萤火虫产生典型的黄绿光进行催化。该反应产生的 560 nm 化学发光在几秒钟内达到峰值，当底物荧光素过量时，光输出与荧光素酶浓度成正比。荧光素酶 (luc) 基因是研究和活性分子筛选的常用报告基因。化学发光技术实际上是无背景的，使得 luc 报告基因成为检测低水平基因表达的理想选择。在标准荧光计数仪中可以可靠地测量到 0.02 pg 的荧光素酶。除了用于基因表达的外，荧光素酶还用于 ATP 的检测。MCE 提供萤火虫荧光素酶 (HY-P1004) 、荧光素游离酸 (HY-12591A) 及其水溶性钠盐 (HY-12591) 和钾盐 (HY-12591B) 。 产品来源： https://www.medchemexpress.cn/D-Luciferin.html 研究领域：Others 作用靶点：Fluorescent Dye 结构分类：其他类 In Vitro: 1. Precautions a) There are three forms of D-luciferin, namely free acid (HY-12591A), potassium salt (HY-12591B) and sodium salt (HY-12591). The sodium salt and potassium salt forms of D-luciferin are easily soluble in aqueous buffers (pH 6.1-6.5). Stock solutions can be made in ATP-free water and stored at -20℃, protect from light. The free acid can be neutralized with DMSO or an appropriate base to dissolve. At a higher pH, luciferin undergoes a base-catalyzed formation of dehydroluciferin, as well as racemization to the L-isomer. b) The D-luciferin can be used with any existing reporter assay or ATP assay system. c) If testing for ATP, minimize all possible sources of ATP contamination by wearing gloves and using ATP-free containers. Use only sterile ATP-free water and reagents. Use autoclaved water for all reagent preparations. 2. Experimental Protocols This protocol only provides a guideline, and should be modified according to your specific needs. 2.1 Example protocol for in vitro bioluminescent image assays a) Prepare D-luciferin stock solution in DMSO. Mix well. Use immediately, or make single use aliquots, and store at -20℃, avoid freeze-thaw cycles, avoid exposure to the light. b) Prepare a 0.5-1 mM working solution of D-Luciferin in pre-warmed tissue culture medium. c) Aspirate media from cultured cells. d) Add D-Luciferin working solution to cells, and incubate the cells for 5-10 minutes at 37℃ just prior to imaging. 2.2 In vivo assays Note: Since the in vivo experimental dose is relatively large, generally 150 mg/kg, it is recommended to choose HY-12591B or HY-12591. 2.3 Example protocol for D-Luciferin reporter assays a) Prepare D-luciferin stock solution in DMSO. Use immediately, or make single use aliquots, and store at -20℃, avoid freeze-thaw cycles, avoid exposure to the light. b) Prepare a 1 mM working solution of D-Luciferin with 3 mM ATP, 1 mM DTT and 15 mM MgSO4 in 25 mM tricine buffer pH 7.8. c) Pipette 5-10 μLof cell lysate into a microplate. Use lysis reagent or buffer without lysate as a blank. d) Prime luminometer with D-Luciferin working solution according to manufacturer’s instructions. e) Inject 200 μL of D-luciferin working solution with no delay and a 10 second integration time. 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE | TMRM Perchlorate 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。