耗材描述
2-NBDG
产品活性:2-NBDG 是一种荧光标记的脱氧葡萄糖类似物,主要用于直接监测活细胞和组织的葡萄糖摄取。它还可用作局部对比剂,用于检测肿瘤。2-NBDG 可用于实时共焦、高分辨率或广场荧光显微镜以及流式细胞术。探针可以被 488 nm 的激光激发,产生环境敏感荧光。光稳定性低于罗丹明荧光探针。
产品来源: https://www.medchemexpress.cn/2-NBDG.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Preparation of 2-NBDG working solution
1.1 Preparation of the stock solution
Dissolve 1 mg of 2-NBDG in 2.92 mL of DDH2O to obtain 1 mM of 2-NBDG.
Note: It is recommended to store the stock solution at -20 ℃ or -80 ℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of 2-NBDG working solution.
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 10-200 μM of 2-NBDG working solution.
Note: Please adjust the concentration of 2-NBDG working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1,000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of 2-NBDG working solution, and then incubate at room temperature for 5-60 minutes.
2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. .If test viability, recorded the optical density (O.D.) at 540/570 nm. Cell viability was calculated as a control ratio and plotted against the logarithmic concentration of the drug to calculate IC50.
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