耗材描述
6-NBDG
产品活性:6-NBDG 是一种荧光葡萄糖类似物,可以用于荧光成像和监测葡萄糖转运和摄取。6-NBDG 可作为荧光探针检测巨噬细胞的动脉硬化斑块。
产品来源: https://www.medchemexpress.cn/6-nbdg.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Incubate sample with RPMI (without glucose) containing a final concentration of 200 μM 6-NBDG.
2. Use a curved forcep to embeds sample into the mixture, and stretches the samplet through a drop of agarose (40°C, 20 μL) places on a pre-heated (40°C) glass slide.
3. Gently place another preheated glass slide on the top of sample, so that the whole sample embeds into the agarose-6-NBDG mixture.
4. Take confocal images with a confocal laser scanning microscope (Ex: 471 nm, Em: 500–600 nm).
相关产品:MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE
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