耗材描述
ADHP
产品活性:ADHP 是一种荧光过氧化物酶底物(λex=530 nm, λem=590 nm)。
产品来源: https://www.medchemexpress.cn/ADHP.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: To obtain the parameters Km and kcat for Compound I, two independent methods are used. Initially, the oxidation of ADHP using the injector functionality built-in to the fluorescence plate reader is studied. The auto-injector dispenses the H2O2 to initiate the reaction, as a means of generating a set of progress curves. Analysis for MPO-mediated oxidation of ADHP gives a Km of 31±4 μM and the kcat of 186± 6 s 1.The kobs also increases over the experimental range of ADHP concentrations from 1 to 80 μM and for the converse experiment holding substrate constant over 3 to 45 nM MPO. The apparent second order rate constant obtain from the slope of kobs against ADHP concentration Kappon is 2.1±0.2 mM/s.
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