rno-miR-547-5p inhibitor 产品活性：rno-miR-547-5p inhibitor 是一种全链经过甲氧基修饰的成熟miRNA的互补单链。miRNA inhibitor通过特异性结合成熟miRNA，阻止miRNA与其靶基因的互补配对，从而抑制miRNA的功能，可用于miRNA 功能缺失性（loss-of-function）研究。 产品来源： https://www.medchemexpress.cn/rno-mir-547-5p-inhibitor.html 研究领域：Epigenetics 作用靶点：MicroRNA In Vitro: 1. miRNA Resuspension 1.1 Briefly centrifuge the tube to ensure that the dried miRNA is at the bottom of the tube. 1.2 Resuspend the miRNA using nuclease free water to generate 20 μM stock solution. For 5 nmol miRNA: add 250 μL nuclease free water. For 20 nmol miRNA: add 1000 μL nuclease free water. 1.3 Aliquot miRNAs into one or more tubes to limit the number of freeze-thaw cycles ( 1.4 Store at or below -20°C or -80°C in a non-frost-free freezer until use. 2. Prepare cells 2.1 Inoculate cells in advance for cell transfection. Note: The viability and general health of cells prior to transfection significantly affect transfection result. 3. Transfection 3.1 Prepare transfection mix A and B. For per well of a 6-well plate: A: 120 μL serum-free medium + 5 μL miRNA inhibitors; B: 121 μL serum-free medium + 4 μL PolyFast Transfection Reagent (HY-K1014). For per well of a 24-well plate: A: 23.75 μL serum-free medium + 1.25 μL miRNA inhibitors; B: 24 μL serum-free medium + 1 μL PolyFast Transfection Reagent (HY-K1014). For per well of a 96-well plate: A: 4.75 μL serum-free medium + 0.25 μL miRNA inhibitors; B: 4.8 μL serum-free medium + 0.2 μL PolyFast Transfection Reagent (HY-K1014). Note: The recommended working concentration is 100nM for miRNA inhibitors. miRNA function can vary greatly, depending on the miRNA, the cell line, and the chosen analysis method. To determine the concentration that provides optimal results, optimization experiments using varying mimic/inhibitor concentrations should be performed. The optimized range suggests changing the miRNA concentration in the range of 20 to 500nM. If other transfection reagents are used, the amount of transfection reagent needs to be adjusted according to the specific situation. 3.2 Mix the diluted PolyFast Transfection Reagent and miRNA gently. Incubate at room temperature for 15 minutes. 3.3 Remove culture medium from cells, wash with PBS. 3.4 Add transfection mix (A+B) to cells. For per well of a 6-well plate: add 1750 μL fresh medium without Pen/Strep, then add 250 μL of the transfection mix (A+B) to the well, and mix well. For per well of a 24-well plate: add 450 μL fresh medium without Pen/Strep, then add 50 μL of the transfection mix (A+B) to the well, and mix well. For per well of a 96-well plate: add 90 μL fresh medium without Pen/Strep, then add 10 μL of the transfection mix (A+B) to the well, and mix well. 3.5 Incubate cells for 1–3 days at 37°C. Then, analyze transfected cells. The medium can be replaced with fresh serum-containing medium after 6 hours if necessary. 相关产品：Camptothecin | Cl-amidine hydrochloride | Diosgenin | Aurintricarboxylic acid | MicroRNA Mimic Negative Control | RGLS4326 | PIN1 inhibitor API-1 | LIN28 inhibitor LI71 | Enoxacin hydrate | MicroRNA Antagomir Negative Control | Targapremir-210 | MicroRNA Inhibitor Negative Control | Pseudoprotodioscin | SID 3712249 | hsa-miR-23a-3p antagomir | hsa-miR-124-3p inhibitor | mmu-miR-155-5p mimic | Cobomarsen | hsa-miR-138-5p agomir | hsa-miR-138-5p antagomir | hsa-miR-146a-5p mimic | hsa-miR-17-5p mimic | hsa-miR-21-5p inhibitor | hsa-miR-21-5p mimic | hsa-miR-26b-5p mimic | hsa-miR-27b-3p mimic | hsa-miR-30c-5p inhibitor | Lin28-let-7a antagonist 1 | MIR96-IN-1 | hsa-miR-106a-3p inhibitor 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。