B7447 小牛血清，澳大利亚来源，γ辐照，无菌，适合细胞培养，适合杂交瘤细胞
B7447 Sigma-Aldrich Bovine Calf Serum
Australia origin, γ-irradiated, sterile-filtered, suitable for cell culture, suitable for hybridoma
别名：Calf Serum
MDL编号：MFCD00243108
属性
生物来源
bovine fetus
无菌性
γ-irradiated
sterile-filtered
形式
liquid
来源
Australia origin
technique(s)
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
储存温度
−20°C
说明
一般描述
Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro.
Bovine Calf Serum (BCS) is collected from calves that are less than 12 months old. The serum contains approximately double the protein of Fetal Bovine Serum (FBS). BCS will promote growth in a variety of mammalian cell lines and is often used as a cost effective substitute for FBS.
Gamma irradiation has been shown to inactivate up to six logs of many biological contaminants in serum while maintaining growth promotion potential, providing added confidence in the quality and performance of the serum. The standard gamma irradiation of Sigma-Aldrich serum includes exposure under controlled conditions to a 60Co source with a delivered dose of 25 - 35 kGy.
动物血清通常用于补充基础培养基配方，以实现多种细胞类型在体外的最佳生长。
小牛血清（BCS）是从12个月以下的小牛身上采集的。该血清的蛋白质含量约为胎牛血清（FBS）的两倍。BCS将促进多种哺乳动物细胞系的生长，通常被用作FBS的经济高效的替代品。
研究表明，γ射线照射可使血清中多达六个对数的许多生物污染物失活，同时保持促进生长的潜力，增加了对血清质量和性能的信心。Sigma-Aldrich血清的标准伽马射线照射包括在受控条件下暴露在60Co源下，剂量为25-35kgy。
储存及稳定性
To effectively preserve the integrity of animal serum, it should be stored frozen and protected from light. Multiple thaw/freeze cycles should be avoided as they will hasten the degradation of serum nutrients and can result in the formation of insoluble precipitates.
For stability and optimal performance, serum should be stored at −20 °C and used prior to the labeled expiration date
为了有效地保存动物血清的完整性，应将其冷冻保存并避光。应避免多次解冻/冷冻循环，因为它们会加速血清营养素的降解，并可能导致不溶性沉淀物的形成。
为了保持稳定性和最佳性能，血清应储存在−20°C，并在标记的有效期之前使用
分析说明
Endotoxin and hemoglobin tested
安全信息
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(F)
Not applicable
闪点(C)
Not applicable
