CodePlex 多重细胞因子蛋白质组芯片-IsoPlexis-艾飕普
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多重细胞因子分泌组蛋白分析 采用 CodePlex 多重细胞因子分泌芯片，只需少量样本，加上完全自动化检测，适合珍贵的样本分析，软件当天即 可提供直观性结果；不需要专门的技术人员操作。
成立于2013年的IsoPlexis公司总部位于美国的康涅狄格州，是一家专注于单细胞多重功能蛋白质检测解决方案的领先供应商。自2018年商业化以来，已在全球成功装机超过200台，是众多国际知名研究机构和制药厂商的合作伙伴，相关文献发布超过100篇，并有多篇高分文章发布在Nature、Cell、Immunity等刊物上。
IsoPlexis致力于提供业内广受赞誉的精准单细胞分析系统，助力加速对抗癌症及各种疑难病症的进程。通过揭示细胞亚群中独特的免疫生物标志物，推动免疫疗法及靶向治疗迈向更精准、个性化的发展阶段。
IsoPlexis采用微流控技术，通过单细胞蛋白组芯片上12,000个微室同时可捕获上千个细胞，并利用每个微室中固定包埋的Barcode抗体条形码技术捕获32种活的细胞因子。同时可以裂解细胞，检测单细胞胞内磷酸化通路。检测完成后，数据会自动传输到分析软件，进行生信分析和可视化数据呈现，并可导出数据图表直接用于文献发表。
IsoPlexis 单细胞功能蛋白质组学分析技术可深入洞察单细胞水平的蛋白质功能性，全自动端对端的 IsoLight 分析系统，利用微流控技术进行单细胞捕获、细胞培养、全自动多重细胞因子检测、高内涵数据读取。自动化的分析系统，节省人力减少误差。搭配 IsoCode 单细胞微流控芯片，进行单个细胞超过30 种细胞因子的检测，数据使用IsoSpeak 多维生信分析软件简单直观地进行多参数系统分析。全自动化的分析技术，揭示单细胞蛋白质组的异质表达，单细胞功能性数据也被多篇文献证明与许多疾病临床预后生存率等成正相关。IsoPlexis 全自动单细胞功能蛋白质组分析系统，加速免疫研究的进展。
IsoPlexis 技术原理
微流控抗体编码技术特异性多重因子检测用于分离活细胞的单细胞微室简单直观的分析软件实验数据可直接用于文献发表 IsoLight/IsoSpark 全自动分析平台，采用微流控技术，搭配IsoCode 单细胞微流控蛋白质组分析芯片，进行单细胞蛋白质组分析。芯片内12,000 个独立亚纳升等级的微室可以同时捕获上千个单细胞，微室底部的独特抗体编码技术可以在单细胞培养于微室时实时捕抓分泌因子或是胞内蛋白，并采集30 种以上的蛋白信号。采集IsoLight/IsoSpark 全自动分析系统会接着进行基于ELISA 原理的蛋白检测，多重蛋白荧光信号通过仪器内成像系统收集后自动传输至IsoSpeak 多维生信分析软件进行数据分析。简单直观的分析软件，不需额外培训的生信分析人员，分析数据图表可以直接应用于文章发表。
场景应用
CodePlex 多重蛋白定量解决方案
只需11μl 样本即可获得有意义的数据
CodePlex 解决方案旨在帮助研究人员实现一种简化而快速的方法来产生大批量多重细胞因子数据。特别的芯片设计与微室抗体编码技术，仅需11μl 样本 ( 单个样本5.5μl，复孔)，即可实现全自动多重因子分析，大幅减少样本用量，缩短人员操作时间。联合IsoCode 单细胞分泌蛋白质组芯片，从样本上清至样本内单细胞分泌蛋白进行完整分析，更全面了解细胞的异质表达。
CodePlex 多重蛋白定量分析
适用物种：人、小鼠、非人灵长类
适用多种样品类型：细胞培养上清、血清/ 血浆、脑脊液、肺泡灌洗液、尿液等
芯片类型：适应性免疫、天然免疫、细胞因子风暴、炎症等因子检测
研究领域：涵盖癌症免疫、细胞治疗、感染免疫、炎症应答、干细胞等
多重细胞因子分泌蛋白质组检测
Human Adaptive Immune 人类适应性免疫应答-22 plex
GM-CSF, Granzyme B, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9,IL-10, IL-13, IL-15, IL-17A, IP-10, MCP-1, MIP-1α, MIP-1β, Perforin,sCD137, TNF-α, TNF-β
Human Innate Immune 人类天然免疫应答-19 plex
EGF, GM-CSF, Granzyme B, IFN-γ, IL-1β, IL-4, IL-6, IL-7, IL-8, IL-10,IL-15, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF-BB, sCD137, TNF-α,VEGF
Human Cytokine Storm Panel 人类细胞因子风暴免疫应答-18 plex
GM-CSF, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-13,IL-17A, IP-10, MCP-1, MIP-1α, MIP-1β, Perforin, TNF-α
Human Stem Cell Signaling 人类干细胞免疫应答-17plex
IL-17A, MIP-1α, IL-6, IL-4, MIP-1β, IL-8, IFN-γ, GM-CSF, IL-10, TNF-α, MCP-1, IL-2, IL-15,RANTES, IL-1α, IL-1β, CXCL5
Human Cancer Signaling 人类肿瘤免疫应答-17 plex
EGF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-13, MCP-1, MIF, PDGF-BB,RANTES, TNF-α
Mouse Adaptive Immune 小鼠适应性免疫应答-16 plex
GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-17A, IP-10, KC, MCP-1, MIP-1α,RANTES, TNF-α
Mouse Innate Immune 小鼠天然免疫应答-16 plex
IFN-γ, TNF-α, MIP-1α, IL-15, GM-CSF, IL-5, IL-10, IL-13, IL-6, IL-17A, MCP-1, IP-10, MIP-1β,EGF, PDGF-BB, MIF
Mouse Inflammation 小鼠炎症应答-16 plex
IFN-γ, TNF-α, MIP-1α, IL-2, IL-5, IL-10, IL-13, IL-4, IL-6, IL-1β, IL-17A, IL-12, MCP-1, IP-10,KC, GM-CSF
Non-Human Primate Adaptive Immune 非人灵长类适应性免疫应答-13plex
GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, TNF-α
Mouse Stem Cell Signaling - 小鼠干细胞免疫应答Coming Soon
GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-15, IL-17A, MCP-1, MIP-1α, MIP-1β,RANTES, TNF-α
订货信息
产品货号 | 产品名称 | 产品描述 |
CODEPLEX-2100-1 | Codeplex Loading Training Chip | - |
CODEPLEX-2L01-1 | CodePlex Adaptive Immune Chip – (H) 1 | - |
CODEPLEX-2L01-2 | CodePlex Adaptive Immune Chip – (H) 2 | - |
CODEPLEX-2L01-4 | CodePlex Adaptive Immune Chip – (H) 4 | - |
CODEPLEX-2L01-6 | CodePlex Adaptive Immune Chip – (H) 6 | - |
CODEPLEX-2L01-8 | CodePlex Adaptive Immune Chip – (H) 8 | - |
CODEPLEX-2L04-1 | CodePlex Adaptive Immune Chip – (M) 1 | - |
CODEPLEX-2L04-2 | CodePlex Adaptive Immune Chip – (M) 2 | - |
CODEPLEX-2L04-4 | CodePlex Adaptive Immune Chip – (M) 4 | - |
CODEPLEX-2L04-6 | CodePlex Adaptive Immune Chip – (M) 6 | - |
CODEPLEX-2L04-8 | CodePlex Adaptive Immune Chip – (M) 8 | - |
CODEPLEX-2L09-1 | CodePlex Adaptive Immune Chip – (NHP) 1 | - |
CODEPLEX-2L09-2 | CodePlex Adaptive Immune Chip – (NHP) 2 | - |
CODEPLEX-2L09-4 | CodePlex Adaptive Immune Chip – (NHP) 4 | - |
CODEPLEX-2L09-6 | CodePlex Adaptive Immune Chip – (NHP) 6 | - |
CODEPLEX-2L09-8 | CodePlex Adaptive Immune Chip – (NHP) 8 | - |
CODEPLEX-2L03-1 | CodePlex Innate Immune Chip – (H) 1 | - |
CODEPLEX-2L03-2 | CodePlex Innate Immune Chip – (H) 2 | - |
CODEPLEX-2L03-4 | CodePlex Innate Immune Chip – (H) 4 | - |
CODEPLEX-2L03-6 | CodePlex Innate Immune Chip – (H) 6 | - |
CODEPLEX-2L03-8 | CodePlex Innate Immune Chip – (H) 8 | - |
CODEPLEX-2L02-1 | CodePlex Cytokine Storm Chip – (H) 1 | - |
CODEPLEX-2L02-2 | CodePlex Cytokine Storm Chip – (H) 2 | - |
CODEPLEX-2L02-4 | CodePlex Cytokine Storm Chip – (H) 4 | - |
CODEPLEX-2L02-6 | CodePlex Cytokine Storm Chip – (H) 6 | - |
CODEPLEX-2L02-8 | CodePlex Cytokine Storm Chip – (H) 8 | - |
CODEPLEX-2L05-1 | CodePlex Intracellular Proteome Tumor Signaling 1 Chip – (H) 1 | - |
CODEPLEX-2L05-2 | CodePlex Intracellular Proteome Tumor Signaling 1 Chip – (H) 2 | - |
CODEPLEX-2L05-4 | CodePlex Intracellular Proteome Tumor Signaling 1 Chip – (H) 4 | - |
CODEPLEX-2L05-6 | CodePlex Intracellular Proteome Tumor Signaling 1 Chip – (H) 6 | - |
CODEPLEX-2L05-8 | CodePlex Intracellular Proteome Tumor Signaling 1 Chip – (H) 8 | - |
产品货号 | 产品名称 | 产品描述 |
PANEL-2L01-1 | CodePlex Adaptive Immune Panel – (H) 1 | - |
PANEL-2L01-2 | CodePlex Adaptive Immune Panel – (H) 2 | - |
PANEL-2L01-4 | CodePlex Adaptive Immune Panel – (H) 4 | - |
PANEL-2L01-6 | CodePlex Adaptive Immune Panel – (H) 6 | - |
PANEL-2L01-8 | CodePlex Adaptive Immune Panel – (H) 8 | - |
PANEL-2L04-1 | CodePlex Adaptive Immune Panel – (M) 1 | - |
PANEL-2L04-2 | CodePlex Adaptive Immune Panel – (M) 2 | - |
PANEL-2L04-4 | CodePlex Adaptive Immune Panel – (M) 4 | - |
PANEL-2L04-6 | CodePlex Adaptive Immune Panel – (M) 6 | - |
PANEL-2L04-8 | CodePlex Adaptive Immune Panel – (M) 8 | - |
PANEL-2L09-1 | CodePlex Adaptive Immune Panel – (NHP) 1 | - |
PANEL-2L09-2 | CodePlex Adaptive Immune Panel – (NHP) 2 | - |
PANEL-2L09-4 | CodePlex Adaptive Immune Panel – (NHP) 4 | - |
PANEL-2L09-6 | CodePlex Adaptive Immune Panel – (NHP) 6 | - |
PANEL-2L09-8 | CodePlex Adaptive Immune Panel – (NHP) 8 | - |
PANEL-2L03-1 | CodePlex Innate Immune Panel – (H) 1 | - |
PANEL-2L03-2 | CodePlex Innate Immune Panel – (H) 2 | - |
PANEL-2L03-4 | CodePlex Innate Immune Panel – (H) 4 | - |
PANEL-2L03-6 | CodePlex Innate Immune Panel – (H) 6 | - |
PANEL-2L03-8 | CodePlex Innate Immune Panel – (H) 8 | - |
PANEL-2L02-1 | CodePlex Cytokine Storm Panel – (H) 1 | - |
PANEL-2L02-2 | CodePlex Cytokine Storm Panel – (H) 2 | - |
PANEL-2L02-4 | CodePlex Cytokine Storm Panel – (H) 4 | - |
PANEL-2L02-6 | CodePlex Cytokine Storm Panel – (H) 6 | - |
PANEL-2L02-8 | CodePlex Cytokine Storm Panel – (H) 8 | - |
PANEL-2L05-1 | CodePlex Intracellular Proteome Tumor Signaling 1 Panel – (H) 1 | - |
PANEL-2L05-2 | CodePlex Intracellular Proteome Tumor Signaling 1 Panel – (H) 2 | - |
PANEL-2L05-4 | CodePlex Intracellular Proteome Tumor Signaling 1 Panel – (H) 4 | - |
PANEL-2L05-6 | CodePlex Intracellular Proteome Tumor Signaling 1 Panel – (H) 6 | - |
PANEL-2L05-8 | CodePlex Intracellular Proteome Tumor Signaling 1 Panel – (H) 8 | - |
产品货号 | 产品名称 | 产品描述 |
CODEPLEX-2100-1 | Codeplex Loading Training Chip | - |
CODEPLEX-2L01-1 | CodePlex Adaptive Immune Chip – (H) 1 | - |
CODEPLEX-2L01-2 | CodePlex Adaptive Immune Chip – (H) 2 | - |
CODEPLEX-2L01-4 | CodePlex Adaptive Immune Chip – (H) 4 | - |
CODEPLEX-2L04-1 | CodePlex Adaptive Immune Chip – (M) 1 | - |
CODEPLEX-2L04-2 | CodePlex Adaptive Immune Chip – (M) 2 | - |
CODEPLEX-2L04-4 | CodePlex Adaptive Immune Chip – (M) 4 | - |
CODEPLEX-2L09-1 | CodePlex Adaptive Immune Chip – (NHP) 1 | - |
CODEPLEX-2L09-2 | CodePlex Adaptive Immune Chip – (NHP) 2 | - |
CODEPLEX-2L09-4 | CodePlex Adaptive Immune Chip – (NHP) 4 | - |
CODEPLEX-2L03-1 | CodePlex Innate Immune Chip – (H) 1 | - |
CODEPLEX-2L03-2 | CodePlex Innate Immune Chip – (H) 2 | - |
CODEPLEX-2L03-4 | CodePlex Innate Immune Chip – (H) 4 | - |
CODEPLEX-2L02-1 | CodePlex Cytokine Storm Chip – (H) 1 | - |
CODEPLEX-2L02-2 | CodePlex Cytokine Storm Chip – (H) 2 | - |
CODEPLEX-2L02-4 | CodePlex Cytokine Storm Chip – (H) 4 | - |
CODEPLEX-2L05-1 | CodePlex Intracellular Proteome Tumor Signaling 1 Chip – (H) 1 | - |
CODEPLEX-2L05-2 | CodePlex Intracellular Proteome Tumor Signaling 1 Chip – (H) 2 | - |
CODEPLEX-2L05-4 | CodePlex Intracellular Proteome Tumor Signaling 1 Chip – (H) 4 | - |
产品货号 | 产品名称 | 产品描述 |
S-PANEL-2L01-1 | CodePlex Adaptive Immune Panel – (H) 1 | - |
S-PANEL-2L01-2 | CodePlex Adaptive Immune Panel – (H) 2 | - |
S-PANEL-2L01-4 | CodePlex Adaptive Immune Panel – (H) 4 | - |
S-PANEL-2L04-1 | CodePlex Adaptive Immune Panel – (M) 1 | - |
S-PANEL-2L04-2 | CodePlex Adaptive Immune Panel – (M) 2 | - |
S-PANEL-2L04-4 | CodePlex Adaptive Immune Panel – (M) 4 | - |
S-PANEL-2L09-1 | CodePlex Adaptive Immune Panel – (NHP) 1 | - |
S-PANEL-2L09-2 | CodePlex Adaptive Immune Panel – (NHP) 2 | - |
S-PANEL-2L09-4 | CodePlex Adaptive Immune Panel – (NHP) 4 | - |
S-PANEL-2L03-1 | CodePlex Innate Immune Panel – (H) 1 | - |
S-PANEL-2L03-2 | CodePlex Innate Immune Panel – (H) 2 | - |
S-PANEL-2L03-4 | CodePlex Innate Immune Panel – (H) 4 | - |
S-PANEL-2L02-1 | CodePlex Cytokine Storm Panel – (H) 1 | - |
S-PANEL-2L02-2 | CodePlex Cytokine Storm Panel – (H) 2 | - |
S-PANEL-2L02-4 | CodePlex Cytokine Storm Panel – (H) 4 | - |
S-PANEL-2L05-1 | CodePlex Intracellular Proteome Tumor Signaling 1 Panel – (H) 1 | - |
S-PANEL-2L05-2 | CodePlex Intracellular Proteome Tumor Signaling 1 Panel – (H) 2 | - |
S-PANEL-2L05-4 | CodePlex Intracellular Proteome Tumor Signaling 1 Panel – (H) 4 | - |