赛默飞Thermo Acclaim 300 C18 Protein and Peptide Column
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液相色谱柱
High-Resolution Separation of Proteins and Peptides
The Acclaim®300 features 3 µm silica particles for rapid analysis of complex protein digests. The Acclaim 300’s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.
Designed for high-resolution peptide mapping and protein separations
High-efficiency 3 µm spherical silica substrate
High-performance bonding chemistry on 300 Å pore silica
Application tested for suitablility in peptide mapping
Reproducible for dependable results
LC/MS compatible
Manufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions.
The use of a 3 µm silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 µm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.
| Acclaim PepMap for TFA-Free Peptide MappingSpecifications | |||
|---|---|---|---|
| Column type | Nano | Capillary | Micro |
| Column i.d. | 75 µm | 300 µm | 1.0 mm |
| Length | 50, 150, 250, and 500 mm | 50, 150, and 250 mm | 50, 150, and 250 mm |
| Recommended Flow Rate | 200 nL/min | 4 µL/min | 40 µL/min |
| Packing Material | C18, 100 Å, 3 µm | C18, 100 Å, 3 µm | C18, 100 Å, 3 µm |
| C18, 300 Å, 5 µm | C18, 300 Å, 5 µm | C18, 300 Å, 5 µm | |
| C8, 100 Å, 3 µm | C8, 100 Å, 3 µm | C8, 100 Å, 3 µm | |
| C8, 100 Å, 5 µm | C8, 100 Å, 5 µm | C8, 100 Å, 5 µm | |
| C4, 300 Å, 5 µm | C4, 300 Å, 5 µm | C4, 300 Å, 5 µm | |
| Column | Fused-silica | Glass Lined Tubing | Stainless steel |
| Column Inlet | 1/16-in. PEEK sleeve adapter (provided) nanoViper (optional) | 1/16-in. universal fitting nanoViper (optional) 50µm i.d. PEEK sheathed fused silica with nanoViper fittings | 1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
| Column Outlet | Fused-silica 25-µm i.d./ 280-µm o.d. | Fused-silica 75-µm i.d./ 280-µm o.d | 1/16-in. PEEK tubing nanoViper (optional) 75µm i.d. PEEK sheathed fused silica with nanoViper fittings |
| MS Outlet | 1-m Length | ||
Cytochrome c tryptic digest.
Comparison of acid additives for peptide analysis.
Capillary LC for peptide mapping.
Protein mixture separated using reversed-phase HPLC.
The standard particle size is nominally 3 µm. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters; standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 µm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.
| Analytical Columns | |
|---|---|
| Acclaim 300, C18, 3 µm Analytical (2.1 x 50 mm) | 060263 |
| Acclaim 300, C18, 3 µm Analytical (2.1 x 150 mm) | 060264 |
| Acclaim 300, C18, 3 µm (3.0 x 150 mm) | 063684 |
| Acclaim 300, C18, 3 µm Analytical (4.6 x 50 mm) | 060265 |
| Acclaim 300, C18, 3 µm Analytical (4.6 x 150 mm) | 060266 |
| Guard Columns | |
|---|---|
| Acclaim 300,C18, 3µm, Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580 | 069690 |
| PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA | 069697 |
| Hardware | |
|---|---|
| Acclaim SST Guard Cartridge Holder V-1 | 059456 |
| Guard to Analytical Column Coupler | 059457 |
| Guard Kit (Holder and Coupler) | 059526 |
| Acclaim SST Guard Cartridge Holder V-2 | 069580 |
| Acclaim Guard Kit (Holder and coupler) V-2 | 069707 |
| Guard to Analytical Column Coupler V-2 | 074188 |
| Micro and Nano Columns | |
|---|---|
| Acclaim PepMap100, C18, 3 μm, 100 Å, 75 μm i.d. × 5 cm, nanoViper | 164567 |
| 75 µm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 µm, 300Å, nanoViper | 164708 |
| 300 µm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 µm, 100Å, nanoViper | 164716 |
| 300 µm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 µm, 300Å, nanoViper | 164718 |
| Acclaim PepMap300, C18, 5 µm, 300 Å Wide Pore, 1.0 mm x 5 cm | 163584 |
| Acclaim PepMap100, C18, 3 μm, 100 Å, 1 mm i.d. × 15 cm, nanoViper | 164572 |