Fast, pH-Stable Separation of Oligosaccharides
A variety of HPLC approaches are proposed for characterizing oligosaccharides, but many prove inadequate for separating complex mixtures and are limited by a lack of specificity and high limits of detection. Dionex has developed an improved HPLC technique for carbohydrate analysis based on anion-exchange chromatography coupled with pulsed amperometric detection. This technique provides high-resolution separations with sensitive and specific detection, without derivatization.
Sensitive, direct detection with no derivatization, using pulsed amperometric detection
Very high-efficiency separations
Separations based on size, charge, degree of branching, and linkage isomerism
Low flow rate saves on eluent consumption
Does not require derivatization
CarboPac®PA200 columns are packed with a hydrophobic, polymeric, pellicular anion-exchange resin stable over the range of pH 0–14. The unique pH-stability of the packing material allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.
The CarboPac PA200 column is the latest addition to the CarboPac family of columns for carbohydrate separations. This column
has been specially developed to provide high resolution separations of charged and neutral oligosaccharides and is the
recommended column for these applications. The CarboPac PA200 columns are packed with a hydrophobic, polymeric, pellicular
anion exchange resin stable over the range of pH 0–14. This unique pH-stability of the packing material allows the use of eluent
compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.
Resin Characteristics:
Particle Size:5.5 µm
Pore Size: microporous (< 10 Å)
Cross-linking:55%
Ion exchange capacity: 35 µeq per column
Latex Characteristics:
Functional Group:quaternary ammonium ion
Latex Diameter: 43 nm
Latex Cross-linking: 6%
Typical Operating Parameters:
pH range: 0–14
Temperature Limit: 4–60 °C
Pressure Limit:3500 psi
Organic Solvent Limit: 100% compatible
Typical eluents: High purity water (18.2 megohm-cm), sodium hydroxide, sodium acetate
