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Product IntroductionBioactivity英文名: Meisoindigo描述: Meisoindigo (Natura-α) 是 Indirubin 的衍生物，可以造成急性髓细胞性白血病细胞周期在 G0/G1 期停滞，并
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Product Introduction
Bioactivity
英文名: Meisoindigo
描述: Meisoindigo (Natura-α) 是 Indirubin 的衍生物，可以造成急性髓细胞性白血病细胞周期在 G0/G1 期停滞，并诱导凋亡，具有高抗肿瘤活性。
激酶实验: GSK3 Scintillation Proximity Assay: The competition experiments are carried out in duplicate with 10 concentrations of the inhibitor in clear-bottomed microtiter plates. The biotinylated peptide substrate, biotin-AAEELDSRAGS(PO3H2)PQL, is added at a final concentration of 2 μM in an assay buffer containing 6 milliunits of recombinant human GSK3 (equal mix of both α and β), 12 mM MOPS, pH 7.0, 0.3 mM EDTA, 0.01% β-mercaptoethanol, 0.004% Brij 35, 0.5% glycerol, and 0.5 μg of bovine serum albumin/25 μl and preincubated for 10-15 min. The reaction is initiated by the addition of 0.04 μCi of [γ-33P]ATP and unlabeled ATP in 50 mM Mg(Ac)2 to a final concentration of 1 μM ATP and assay volume of 25 μl. Blank controls without peptide substrate are used. After incubation for 20 min at room temperature, each reaction is terminated by the addition of 25 μl of stop solution containing 5 mM EDTA, 50 μM ATP, 0.1% Triton X-100, and 0.25 mg of streptavidin-coated SPA beads corresponding to ～35 pmol of binding capacity. After 6 h the radioactivity is determined in a liquid scintillation counter. Inhibition curves are analyzed by non-linear regression using GraphPad Prism.
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 18.33 mg/mL (66.36 mM)
关键字: NB4 | G0/G1 | Natura-a | AML | Apoptosis | antitumor | Indirubin | U937 | myeloid | Natura-alpha | phase | inhibit | Meisoindigo | HL-60 | acute | leukemia | Inhibitor | primary
相关产品: Jaceosidin | PARP1/2/TNKS1/2-IN-1 | AZD5582 TFA | TBHQ | Mevastatin | Deracoxib | MC2590 | Ibandronate sodium monohydrate | Rosmarinic acid | 3,4-Dimethoxycinnamic acid
相关库: Anti-Tumor Natural Product Library | Drug Repurposing Compound Library | Anti-Cancer Compound Library | Anti-Cancer Drug Library | Selected Plant-Sourced Compound Library | Anti-Cancer Clinical Compound Library | Anti-Cancer Approved Drug Library | Anti-Cancer Active Compound Library | Natural Product Library for HTS | RO5 Drug-like Natural Product Library
Bioactivity
英文名: Meisoindigo
描述: Meisoindigo (Natura-α) 是 Indirubin 的衍生物，可以造成急性髓细胞性白血病细胞周期在 G0/G1 期停滞，并诱导凋亡，具有高抗肿瘤活性。
激酶实验: GSK3 Scintillation Proximity Assay: The competition experiments are carried out in duplicate with 10 concentrations of the inhibitor in clear-bottomed microtiter plates. The biotinylated peptide substrate, biotin-AAEELDSRAGS(PO3H2)PQL, is added at a final concentration of 2 μM in an assay buffer containing 6 milliunits of recombinant human GSK3 (equal mix of both α and β), 12 mM MOPS, pH 7.0, 0.3 mM EDTA, 0.01% β-mercaptoethanol, 0.004% Brij 35, 0.5% glycerol, and 0.5 μg of bovine serum albumin/25 μl and preincubated for 10-15 min. The reaction is initiated by the addition of 0.04 μCi of [γ-33P]ATP and unlabeled ATP in 50 mM Mg(Ac)2 to a final concentration of 1 μM ATP and assay volume of 25 μl. Blank controls without peptide substrate are used. After incubation for 20 min at room temperature, each reaction is terminated by the addition of 25 μl of stop solution containing 5 mM EDTA, 50 μM ATP, 0.1% Triton X-100, and 0.25 mg of streptavidin-coated SPA beads corresponding to ～35 pmol of binding capacity. After 6 h the radioactivity is determined in a liquid scintillation counter. Inhibition curves are analyzed by non-linear regression using GraphPad Prism.
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 18.33 mg/mL (66.36 mM)
关键字: NB4 | G0/G1 | Natura-a | AML | Apoptosis | antitumor | Indirubin | U937 | myeloid | Natura-alpha | phase | inhibit | Meisoindigo | HL-60 | acute | leukemia | Inhibitor | primary
相关产品: Jaceosidin | PARP1/2/TNKS1/2-IN-1 | AZD5582 TFA | TBHQ | Mevastatin | Deracoxib | MC2590 | Ibandronate sodium monohydrate | Rosmarinic acid | 3,4-Dimethoxycinnamic acid
相关库: Anti-Tumor Natural Product Library | Drug Repurposing Compound Library | Anti-Cancer Compound Library | Anti-Cancer Drug Library | Selected Plant-Sourced Compound Library | Anti-Cancer Clinical Compound Library | Anti-Cancer Approved Drug Library | Anti-Cancer Active Compound Library | Natural Product Library for HTS | RO5 Drug-like Natural Product Library
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