Product Introduction
Bioactivity


英文名: Rostafuroxin

描述: Rostafuroxin (PST 2238) 是洋地黄毒苷衍生物，是一种口服有效的 Na+,K+-ATPase 抑制剂，具有抗高血压和抗 RSV 活性，用于研究治疗原发性高血压的试验。

激酶实验: Kinase assay: Activity assays are conducted using Lance Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) technology from Perkin-Elmer. Briefly, 10 ng/mL FLT3 enzyme, a serial diluted G-749, 80 nM substrate of ULight-poly-GT peptide and variable amounts of ATP (8.5 μM to 1088 μM) are mixed in kinase assay buffer (50 mM HEPES pH 7.5, 10 mM MgCl2, 1 mM EGTA, 2 mM DTT and 0.01% Tween-20) and are added to a 384-well OptiPlate-384 in a volume of 10 μL. Kinase reactions are incubated at room temperature for up to 1 h and then stopped by the addition of 5 μL of 10 mM EDTA. A volume of 5 μL of the specific Eu-labeled-anti-phosphopeptide antibody diluted in LANCE Detection Buffer is then added to a final concentration of 2 nM. After 30-minute incubation, assay plates are incubated at 23°C and the LANCE signal is measured on an EnVision Multilabel Reader. Excitation wavelength is set at 320 nm and emission monitored at 615 nm (donor) and 665 nm (acceptor). The IC50 is calculated using nonlinear regression analysis analysis by GradPad Prism 5.

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 50 mg/mL (133.5 mM)


关键字: ATP1A1 | RSV | Respiratory syncytial virus | Na+/K+ ATPase | phosphorylation | inhibit | PST-2238 | Tyr845 | Sodium potassium pump | EGFR | PST2238 | Rostafuroxin | antihypertensive | RSV-GFP | Inhibitor | digitoxigenin | Ouabain

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相关库: Ion Channel Inhibitor Library | Drug Repurposing Compound Library | Anti-Cancer Drug Library | Clinical Compound Library | Membrane Protein-targeted Compound Library | Anti-Cancer Clinical Compound Library | Inhibitor Library | Orally Active Compound Library | Anti-Viral Compound Library | NO PAINS Compound Library