Product Introduction
Bioactivity


英文名: SRI-37240

描述: SRI-37240 is a powerful inhibitor of premature termination codons (PTCs). It effectively suppresses CFTR nonsense mutations and induces changes in cellular translation termination at PTCs in HEK293T cells. Additionally, when combined with G418, SRI-37240 restores CFTR function in primary bronchial epithelial cells [1].

体外活性: SRI-37240 (1, 3, 10 and 30 μM; 48 h) induces concentration-dependent increases in CFTR-dependent ( Forskolin -stimulated and sensitive to the inhibitor CFTR Inh -172) chloride conductance [1]. SRI-37240 (10 μM; 72 h) significantly increases the amount of full-length, fully glycosylated form of CFTR protein, and the unprocessed, immature form of full-length CFTR protein in 16HBEge cells when co-treated with G418 (100 μM) [1]. SRI-37240 (10 μM; 24 h) alters cellular translation termination at PTCs in HEK293T cells, also increases global densities of ribosomes at normal stop codons without affecting densities of ribosomes in 3-UTRs [1]. SRI-37240 (10 μM; 72 h) restores CFTR function in primary bronchial epithelial cells when combination with G418 [1]. Western Blot Analysis [1] Cell Line: CFTR -G542X 16HBEge Concentration: 10 μM Incubation Time: 24 h Result: Significantly increased the amount of Band C CFTR protein, which represents the full-length, fully glycosylated form of CFTR and Band B, which represents the unprocessed, immature form of full-length CFTR protein when combined with G418 (100 μM).

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year