化合物AP-III-a4 T6759
面议
- TargetMol
- 生产商
- 进口
- 美国
- 现货
- 全国
- 6
- 0
Product IntroductionBioactivity英文名: AP-III-a4描述: AP-III-a4 (ENOblock) 是第一个非底物类似物，可直接与烯醇酶结合，IC50=0.576 uM，可阻碍体内癌细胞转移。细胞实验:
AI问答
配套的仪器设备？
可以搭配的相关耗材试剂？
耗材描述
Product Introduction
Bioactivity
英文名: AP-III-a4
描述: AP-III-a4 (ENOblock) 是第一个非底物类似物，可直接与烯醇酶结合，IC50=0.576 uM，可阻碍体内癌细胞转移。
细胞实验: HCT116 cells(3 x 10E5) are seeded in a 6 well plate. 24 h later, cells are treated with AP-III-a4 (5 μM) for 24hours.
激酶实验: Enolase activity assay is in the 2.0 mM MgSO4 and 400 mM KCl in the absence or presence of ENOblock or NaF, at 37°C by incubating pure enolase (3–9 U) in a buffer containing 50 mM imidazole-HCl (pH 6.8). The reaction is initiated by adding 1 μmol of 2-phospho-D-glycerate, and the OD is measured after 10 min of reaction time with a spectrophometer at 240 nm.
动物实验: In HCT116-xenotransplanted zebrafish tumor xenograft model, were treated with AP-III-a4 of 10 μM.
体外活性: AP-III-a4 induces glucose uptake and inhibits phosphoenolpyruvate carboxykinase (PEPCK) expression in Huh7 hepatocytes and HEK kidney cells. AP-III-a4 induces cell death under hypoxia, and inhibits cancer cell migration and invasion by down-regulation of AKT and Bcl-xL expression in HCT116 cells.
体内活性: AP-III-a4 (10 μM) inhibits cancer cell migration and invasion processes in HCT116-xenotransplanted zebrafish tumor model. In vivo, AP-III-a4 (10 μM) also inhibits adipogenesis and foam cell formation, and causes downregulation of PEPCK expression and induction of glucose uptake.
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 30 mg/mL (47.53 mM)
H2O : < 1 mg/mL (insoluble or slightly soluble)
关键字: Inhibitor | AP-III-a-4 | Phosphopyruvate hydratase | Apoptosis | inhibit | antidiabetic | anticancer | HCT116 | Enolase | Huh7 | zebrafish | APIIIa4 | HEK | AP-III-a4 | AP III a4
相关产品: AM-2394 | KAN0438757 | PFK-015 | ML251 | MitoPQ | 3PO | PF-04937319 | PFK-158 | Globalagliatin | ML266
相关库: Glycometabolism Compound Library | Anti-Cancer Compound Library | Bioactive Compounds Library Max | Kinase Inhibitor Library | Inhibitor Library | Anti-Cancer Active Compound Library | Metabolism Compound Library | NO PAINS Compound Library | Glycolysis Compound Library | Bioactive Compound Library
Bioactivity
英文名: AP-III-a4
描述: AP-III-a4 (ENOblock) 是第一个非底物类似物，可直接与烯醇酶结合，IC50=0.576 uM，可阻碍体内癌细胞转移。
细胞实验: HCT116 cells(3 x 10E5) are seeded in a 6 well plate. 24 h later, cells are treated with AP-III-a4 (5 μM) for 24hours.
激酶实验: Enolase activity assay is in the 2.0 mM MgSO4 and 400 mM KCl in the absence or presence of ENOblock or NaF, at 37°C by incubating pure enolase (3–9 U) in a buffer containing 50 mM imidazole-HCl (pH 6.8). The reaction is initiated by adding 1 μmol of 2-phospho-D-glycerate, and the OD is measured after 10 min of reaction time with a spectrophometer at 240 nm.
动物实验: In HCT116-xenotransplanted zebrafish tumor xenograft model, were treated with AP-III-a4 of 10 μM.
体外活性: AP-III-a4 induces glucose uptake and inhibits phosphoenolpyruvate carboxykinase (PEPCK) expression in Huh7 hepatocytes and HEK kidney cells. AP-III-a4 induces cell death under hypoxia, and inhibits cancer cell migration and invasion by down-regulation of AKT and Bcl-xL expression in HCT116 cells.
体内活性: AP-III-a4 (10 μM) inhibits cancer cell migration and invasion processes in HCT116-xenotransplanted zebrafish tumor model. In vivo, AP-III-a4 (10 μM) also inhibits adipogenesis and foam cell formation, and causes downregulation of PEPCK expression and induction of glucose uptake.
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 30 mg/mL (47.53 mM)
H2O : < 1 mg/mL (insoluble or slightly soluble)
关键字: Inhibitor | AP-III-a-4 | Phosphopyruvate hydratase | Apoptosis | inhibit | antidiabetic | anticancer | HCT116 | Enolase | Huh7 | zebrafish | APIIIa4 | HEK | AP-III-a4 | AP III a4
相关产品: AM-2394 | KAN0438757 | PFK-015 | ML251 | MitoPQ | 3PO | PF-04937319 | PFK-158 | Globalagliatin | ML266
相关库: Glycometabolism Compound Library | Anti-Cancer Compound Library | Bioactive Compounds Library Max | Kinase Inhibitor Library | Inhibitor Library | Anti-Cancer Active Compound Library | Metabolism Compound Library | NO PAINS Compound Library | Glycolysis Compound Library | Bioactive Compound Library
猜你喜欢
感兴趣产品