Product Introduction
Bioactivity


英文名: α-Cyano-4-hydroxycinnamic acid

描述: α-Cyano-4-hydroxycinnamic acid (α-Cyano-4-hydroxycinnamic acid(α-CHCA)) 是非竞争性单羧酸盐转运蛋白 (MCTs) 抑制剂，可抑制线粒体丙酮酸转运蛋白，Ki 值为 6.3 μM。它可作为基质辅助激光解吸电离-飞行时间质谱 (MALDI-TOF) 分析的基质，可以促进肽离子化。

细胞实验: Cells were plated into 96-well plates, at a density of 3 × 10^3 cells per well.?The effect of treatment with CHC (0.6–12 mM) on cell number (total biomass) was determined at 24, 48, and 72 h by the?sulforhodamine B assay (SRB, TOX-6).?IC50 values (CHC concentration that corresponds to 50% of cell growth inhibition) were estimated from 3 independent experiments, each one in triplicate, using GraphPad Software.?Cell proliferation assay was performed as previously described32 and assessed under the treatment conditions previously described, for 5 mM and 10 mM of CHC.?After CHC treatment, cells were incubated with BrdU and its incorporation was assessed at 450 nm (λref = 655 nm) .?Cell growth (total biomass) and cell proliferation for glioma siMCT1 cells and the effect of CHC (1.25–15 mM) on cell number were performed[1].

体外活性: The effectiveness of CHC in glioma cells appears to be dependent on Monocarboxylate transporters (MCTs) membrane expression.?MCT1 downregulation showed similar effects on different glioma cells, supporting CHC as an MCT1 inhibitor.?There was a synergistic effect when combining CHC with temozolomide treatment in U251 cells.?In the CAM in vivo model, CHC decreased the size of tumors and the number of blood vessels formed[1].

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 37mg/mL(195.59mM)


关键字: matrix-assisted | MALDI-TOF | matrix | Inhibitor | lactate | a-Cyano-4-hydroxycinnamic acid | applications | MCTs | pyruvate | laser | desorption | spectrometry | ionization | Monocarboxylate Transporter | a-Cyano-4-hydroxycinnamic acid(a-CHCA) | transporter | α Cyano 4 hydroxycinnamic acid | peptide | inhibit | alpha-Cyano-4-hydroxycinnamic acid | alpha-Cyano-4-hydroxycinnamic acid(alpha-CHCA) | α-Cyano-4-hydroxycinnamic acid | αCyano4hydroxycinnamic acid | facilitate

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相关库: Ion Channel Inhibitor Library | Mitochondria-Targeted Compound Library | Anti-Cancer Compound Library | Membrane Protein-targeted Compound Library | Bioactive Compounds Library Max | Inhibitor Library | Cuproptosis Compound Library | Target-Focused Phenotypic Screening Library | Covalent Inhibitor Library | NO PAINS Compound Library