产品性能
凝集素：56种
一张芯片上的点阵数目：12
点样环境：温度4-8℃，湿度30%~40%
芯片基片：晶芯高分子三维基片，尺寸为75*25 mm
点直径：~120微米
样品重复：每个样品重复3个点；
点间距：X向270微米，Y向300微米（圆心距）
点体积：0.5~1 nl/点
芯片点制后处理：4℃过夜固定后-80℃长期保存
产品应用
1. 特定蛋白表面糖谱构建及差异检测
2. 活细胞表面糖谱构建及差异检测
3. 血清或细胞裂解液糖谱构建及差异检测
4. 糖生物标志物的发现
发表文章
1.Shu-Min Zhou , et al. Lectin RCA-I specifically binds to metastasis-associated cell surface glycans in triple-negative breast cancer. Breast Cancer Research. (2015):1465-5411.[PubMed]
2.Tao SC et al. (2008). Lectin microarrays identify cell-specific and functionally significant cell surface glycan markers. Glycobiology 18(10):761-9. doi: 10.1093/glycob/cwn063. Epub Jul 14. [PubMed]
3.Sun Y, Cheng L, Gu Y, et al. A Human Lectin Microarray for Sperm Surface Glycosylation Analysis[J]. Molecular & Cellular Proteomics, 2016: mcp. M116. 059311. [PubMed]
5. Roy B, et al. On-chip lectin microarray for glycoprofiling of different gastritis types and gastric cancer. Biomicrofluidics. 2014 Jun 6;8(3):034107. [PubMed]
6. Wu J , et al. Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC−MS/MS. J Proteome Res. 2012 Sep 7;11(9):4541-52.[PubMed]
7. Li Y, et al. Detection and verification of glycosylation patterns of glycoproteins from clinical specimens using lectin microarrays and lectin-based immunosorbent assays. Anal Chem. 2011 Nov 15;83(22):8509-16.[PubMed]
8. Zhou SM et al. Lectin microarrays: a powerful tool for glycan-based biomarker discovery. Comb Chem High Throughput Screen. 2011 Sep;14(8):711-9.[PubMed]
9. Li Y , et al. High-throughput lectin microarray-based analysis of live cell surface glycosylation. Curr Protoc Protein Sci. 2011 Feb;Chapter 12:Unit12.9. [PubMed]
