产品名称：Recombinant Human IGF-I/IGF-1 Protein

规格或纯度：无载体, 生物活性, ActiBioPure™, 高性能, ≥90%(SDS-PAGE), 见COA

生化机理：Insulin-like growth factor 1 (IGF-1 or IGF-I), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate Cterminal propeptides. These isoforms are differentially expressed by various tissues. The 7.6 kDa mature IGF1 is identical between isoforms and is generated by proteolytic removal of the N- and Cterminal regions. Mature human IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF2. Circulating IGF-1 is produced by hepatocytes, while local IGF-1 is produced by many other tissues in which it has paracrine effects. IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally. IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism. It plays an important role in muscle regeneration and tumor progression. IGF-1 binds IGF1R, IGF2R, and the insulin receptor, although its effects are mediated primarily by IGF1R. IGF-1 association with IGF binding proteins increases its plasma halflife and modulates its interactions with receptors.

应用：SDS-PAGE, Cell Culture, Functional Studies

生物活性：Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 28.6 ng/mL.

氨基酸：49-118 aa

无载体：Yes

无动物源：No

Accession #：P05019

预测分子量：7.7 kDa

SDS-PAGE：10.2 & 13.8 kDa, under reducing conditions; 10.4 & 13.8 & 20.9 kDa, under non-reducing conditions.

级别：无载体, 生物活性, ActiBioPure™, 高性能

储存缓冲液：Lyophilized from PBS, pH 7.4

浓度：见COA

复溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存温度：-20°C储存,避免反复冻融

运输条件：超低温冰袋运输

稳定性与储存：Store at -20°C for 1 year. Avoid freeze/thaw cycle.

Accession #：P05019

生物活性：Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 28.6 ng/mL.

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