基本描述
产品名称:Recombinant Human Holo-Transferrin
规格或纯度:无动物源,无载体,生物活性,ActiBioPure™,低内毒素,高性能,用于细胞培养,≥95%(SDS-PAGE),铁含量(Iron content)>1000 ppm
应用:SDS-PAGE, Cell Culture, Functional Studies
产品介绍:
转铁蛋白(TF)是血浆中主要的含铁蛋白质,不仅参与铁的运输与代谢,参与呼吸、细胞增殖和免疫系统的调节,更具有抗氧化和杀菌的功能,在细胞培养中具有广泛应用。转铁蛋白以三种形态存在:脱铁(不结合铁离子),饱和铁(结合两分子铁离子)和部分饱和。 本产品为全铁转铁蛋白,即饱和铁状态,除发挥转铁作用外,自身即可作为细胞的铁源,无需在培养基中添加外源性铁。
应用领域:可用于动物细胞培养。
注意事项:本产品仅用于科研、实验室和生产使用,非临床治疗使用,不得用于动物及人体。
生物活性:1、 Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is 0.5-2 μg/mL. 2、Measured in a cell proliferation assay using K562 human chronic myelogenous leukemia erythroleukemic cells. The ED50 for this effect is typically 21.02 ng/mL.
氨基酸:20-698 aa
无载体:Yes
无动物源:Yes
Accession #:P02787
预测分子量:75.2 kDa
SDS-PAGE:75.2 kDa
级别:无动物源, 无载体, 生物活性, ActiBioPure™, 低内毒素, 高性能, 用于细胞培养
产品规格参数
储存缓冲液:Lyophilized from a 0.2 um filtered solution in sterile water.
浓度:铁含量(Iron content)>1000 ppm
复溶:It is recommended to redissolve in sterile deionized water.
储存条件:2-8°C储存
运输条件:低温运输
稳定性与储存:36 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ to -80℃ under sterile conditions after reconstitution. 7-10 days at 2℃ to 8℃ under sterile conditions after reconstitution. Upon delivery aliquot. Avoid freeze/thaw cycle.
功能和特点
Accession #:P02787
生物活性:1、 Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is 0.5-2 μg/mL. 2、Measured in a cell proliferation assay using K562 human chronic myelogenous leukemia erythroleukemic cells. The ED50 for this effect is typically 21.02 ng/mL.
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