产品名称：Recombinant Human CCNB1 Protein

规格或纯度：无载体, ≥90%(SDS-PAGE), 见COA

生化机理：Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis. CCNB1 creates a complex with p34(cdc2) to form the maturation-promoting factor (MPF). CCNB1 is vital for the control of the cell cycle at the G2/M (mitosis) transition. CCNB1 builds up steadily during the G2 and is immediately destroyed at mitosis. The 2 alternative transcripts produce a constitutively expressed transcript and a cell cycle-regulated transcript which is expressed predominantly during G2/M phase. These transcripts are a result of alternate transcription initiation sites. Post-translational: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction (PubMed:16009132). Deubiquitinated by USP22 during G2/M phase (PubMed:27030811). Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

应用：SDS-PAGE, Functional Studies

氨基酸：2-91 aa

无载体：Yes

无动物源：No

Accession #：P14635

预测分子量：10.6 kDa

SDS-PAGE：12.2 kDa, under reducing conditions; 12.2 kDa, under non-reducing conditions.

级别：无载体

储存缓冲液：20mM Tris, 150mM NaCl, 5% Glycerol, pH 8.0

浓度：见COA

储存温度：-20°C储存,避免反复冻融

运输条件：超低温冰袋运输

稳定性与储存：-20℃ 存储12个月；收货后建议分装，避免反复冻融。

Accession #：P14635

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