伯乐ANNEXIN V:APC ASSAY KIT ANNEX50APC
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ANNEXIN V:APC ASSAY KITANNEX50APC抗体英文名：ANNEXIN V:APC ASSAY KIT应用范围：F宿主：N/A标记物：APC克隆性：Kits免疫原：ANNEXIN V KIT规格：50 TESTSThis
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ANNEXIN V:APC ASSAY KIT
- 抗体英文名：ANNEXIN V:APC ASSAY KIT
- 应用范围：F
- 宿主：N/A
- 标记物：APC
- 克隆性：Kits
- 免疫原：ANNEXIN V KIT
- 规格：50 TESTS
This test employs the property of Annexin V to bind to the membrane phospholipid phosphatidylserine (PS) in the presence of Ca2+. PS is exposed at the cell surface during the early stages of apoptosis. Detection of PS is a very sensitive method for detecting cells entering apoptosis, at a time point considerably ahead of nuclear changes such as DNA degradation.
The conjugation protocol used to prepare this product has not changed the native phospholipid binding properties of Annexin V. This protocol is designed to measure apoptosis easily and quickly in a sample of suspended cells.
Storage InformationThis product is shipped at ambient temperature.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. ApplicationsThis product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.Application Name Verified Min Dilution Max Dilution Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Instructions For Use
ANNEX50APC
1) Dilute the10X binding buffer to 1X in distilled water (1 ml binding buffer + 9 ml distilled water).
2) Wash cells once in PBS by gentle shaking or pipetting up and down. Then wash once in 1X binding buffer.
3) Resuspend cells in 1X binding buffer, adjusting to a cell density of 1-5 x 106 cells/ml.
4) Add 5 μl of APC conjugated Annexin V to 100 μl of the cell suspension.
5) Mix and incubate for 10 to15 minutes at room temperature.
6) Wash cells in 1X binding buffer.
7) Resuspend cells in 200 μl of 1X binding buffer.
8) Add 5μl of Propidium Iodide staining solution.
9) Analyse by flow cytometry within 4 hours. Store at 2-8°C in the dark.
This product is shipped at ambient temperature.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
ApplicationsThis product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.Application Name Verified Min Dilution Max Dilution Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Instructions For Use
ANNEX50APC
1) Dilute the10X binding buffer to 1X in distilled water (1 ml binding buffer + 9 ml distilled water).
2) Wash cells once in PBS by gentle shaking or pipetting up and down. Then wash once in 1X binding buffer.
3) Resuspend cells in 1X binding buffer, adjusting to a cell density of 1-5 x 106 cells/ml.
4) Add 5 μl of APC conjugated Annexin V to 100 μl of the cell suspension.
5) Mix and incubate for 10 to15 minutes at room temperature.
6) Wash cells in 1X binding buffer.
7) Resuspend cells in 200 μl of 1X binding buffer.
8) Add 5μl of Propidium Iodide staining solution.
9) Analyse by flow cytometry within 4 hours. Store at 2-8°C in the dark.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
ANNEX50APC
1) Dilute the10X binding buffer to 1X in distilled water (1 ml binding buffer + 9 ml distilled water).
2) Wash cells once in PBS by gentle shaking or pipetting up and down. Then wash once in 1X binding buffer.
3) Resuspend cells in 1X binding buffer, adjusting to a cell density of 1-5 x 106 cells/ml.
4) Add 5 μl of APC conjugated Annexin V to 100 μl of the cell suspension.
5) Mix and incubate for 10 to15 minutes at room temperature.
6) Wash cells in 1X binding buffer.
7) Resuspend cells in 200 μl of 1X binding buffer.
8) Add 5μl of Propidium Iodide staining solution.
9) Analyse by flow cytometry within 4 hours. Store at 2-8°C in the dark.
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