This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page. | Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry 1 | ✓ | | |
| Immunofluorescence | ✓ | | |
| Immunohistology - Frozen | ✓ | | |
| Immunohistology - Paraffin 2 | ✓ | | |
| Immunoprecipitation | ✓ | | |
| Western Blotting | ✓ | 1/500 | 1/2000 |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Histology Positive Control Tissue
- Human appendix
