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The iScript reverse transcription supermix for RT-qPCR is a simple, fast, and sensitive first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. In one tube, the preblended 5x supermix contains all the necessary components except RNA template for reverse transcription.
- Increase reproducibility of reverse transcription and reduce errors during setup — 1-tube format for simple and fast setup and reduced pipetting variability
- Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly effcient RNase H+ MMLV reverse transcriptase
- Use with dilute RNA samples — 5x concentration of supermix allows for greater volume of RNA sample in a 20 µl cDNA synthesis reaction
- Eliminate freeze/thaw cycles — liquid format at –20ºC shortens overall processing time and maximizes stability
- Validate purity of input RNA and gene expression results — optional no-RT controls for detecting genomic DNA contamination
- Obtain accurate results — potent blend of RNase inhibitor prevents RNA degradation during reaction setup and reverse transcription
- Increase primer design flexibility and prevent 5' and 3' bias — optimum blend of oligo(dT) and random primers in the 5x supermix for complete RNA coverage
- Complete cDNA synthesis and qPCR the same day — fast 26 minute cDNA synthesis protocol
用于 RT-qPCR 的 iScript 逆转录试剂盒是一种简单、快速、灵敏的第一链 cDNA 合成试剂盒，它使用实时 qPCR 进行基因表达分析。 在一个试管中，预混合的 5x 超混合液包含所有必要组分，除了用于逆转录的 RNA 模板。
提高逆转录的可重复性并减少反应设置步骤的错误 — 单管装试剂用于简单和快速的反应设置并减少了移液误差 检测低丰度靶基因并在基因表达分析过程中稳定 RNA — RNA 上样的线性动态范围广泛 (1 µg–1 pg)，使用高效的 RNase H+ MMLV 逆转录酶 适用于稀浓度的 RNA 样品 — 5x 浓度的预混液允许在 20 µl cDNA 合成反应中使用更大体积的 RNA 样本 无需反复冻融 — –20ºC 的液体形式缩短了总处理时间并获得了最大的稳定性 验证上样 RNA 的纯度和基因表达结果 — 含用于检测基因组 DNA 污染的无RT对照 获得准确结果 — RNase 抑制剂的强效混合物可在反应设置和逆转录过程中阻止 RNA 降解 提高引物设计灵活性并防止出现 5' 和 3' 偏差 — 很好地混合了 5x 预混液中的低聚糖 (dT) 和随机引物，以实现完整的 RNA 覆盖 同一天完成 cDNA 合成和 qPCR — 40 分钟 cDNA 快速合成实验方案
