准备最终体积 80% ~ 90% 的配制用水（超纯水、注射用水等）。称取与配制量相对应的粉末培养基（13.5 g/L）。温和搅拌配制用水，边搅拌边加入称好的粉末培养基，持续搅拌至完全溶解。称取所需量的碳酸氢钠，添加到培养基中，持续搅拌至完全溶解。测量培养基的 pH 值，若与目标值不同，则使用 1 N 的盐酸或 1 N 的氢氧化钠水溶液调整 pH 值。加入配制用水至最终容量，搅拌数分钟使其均匀混合。此时若过度搅拌可能会导致 pH 值升高。使用孔径为 0.1 μm 或 0.22 μm 的滤膜进行过滤，无菌分装至灭菌容器中。分装后的培养基请保存在 2 ~ 8°C 的阴凉避光处。
Sodium chloride................................................ 6,400 mg
Potassium Chloride............................................... 400 mg
Calcium Chloride (anhydrate)................................... 200 mg
Magnesium sulfate (Anhydrate).................................. 97.7 mg
Sodium Dihydrogen Phosphate (Dihydrate)..................... 125 mg
Iron(III)Nitrate (Enneahydrate)................................. 0.1 mg
Glucose............................................................. 4,500 mg
Sodium Pyruvate................................................... 110 mg
L-Arginine Hydrochloride....................................... 84 mg
L-Cystine Dihydrochloride....................................... 62.6 mg
Glycine............................................................. 30 mg
L-Histidine Hydrochloride Hydrate............................. 42 mg
L-Isoleucine....................................................... 104.8 mg
L-Leucine.......................................................... 104.8 mg
L-Lysine Hydrochloride.......................................... 146.2 mg
L-Methionine....................................................... 30 mg
L-Phenylalanine.................................................. 66 mg
L-Serine............................................................ 42 mg
L-Threonine....................................................... 95.2 mg
L-Tryptophan...................................................... 16 mg
L-Tyrosine Disodium............................................. 89.5 mg
L-Valine........................................................... 93.6 mg
Choline Tartrate.................................................. 7.2 mg
Folic Acid.......................................................... 4 mg
Nicotinamide..................................................... 4 mg
Calcium Pantothenate........................................... 4 mg
Pyridoxal Hydrochloride........................................ 4 mg
Riboflavin......................................................... 0.4 mg
Thiamine Hydrochloride........................................ 4 mg
Myo-nositol...................................................... 7.2 mg
Phenol Red......................................................... 15 mg
L-Glutamine....................................................... 584 mg
