Ham's F12 Medium 是根据 Richard G. Ham 于 1965 年报告的液体合成培养液配方，经过我们公司特殊的制备方法调制而成的粉末培养基。
该培地非常适用于哺乳动物细胞的单细胞培养，并广泛用于 CHO 细胞的培养。此外，它还表现出在人类细胞以及各种细胞的原代培养中具有优良的增殖支持能力。
称取 10.6g 该粉末培养基溶解于蒸馏水中，制成 1000mL 溶液。在此过程中，温度不要超过 37 摄氏度。加入适量碳酸氢钠（加入 1.2-2.0g 后，在 5% CO₂ 下，37℃时 pH 值为 7.1-7.4），溶解后立即进行过滤灭菌。预先使用 CO₂ 气体调节 pH 值后再进行过滤可以防止过度的 pH 升高。如不立即使用，应密封保存在阴凉处（2-10℃）。在使用前，根据需要加入适量的血清。
Sodium Chloride................................ 7,599 mg
Potassium Chloride............................. 224 mg
Calcium Chloride(Anhydrate).................. 33.31 mg
Magnesium Chloride (Hexahydrate)............. 122 mg
Disodium Hydrogen Phosphate(Dihydrate)...... 177.97 mg
Copper Sulfate (II) (Pentahydrate)............. 0.0025 mg
Zinc Sulfate(Heptahydrate)..................... 0.863 mg
Iron(II)Sulfate(Heptahydrate).................. 0.834 mg
Glucose.......................................... 1,802 mg
L-Arginine Hydrochloride...................... 211 mg
L-Alanine....................................... 8.91 mg
L-Asparagine(Monohydrate).................... 15.01 mg
L-Aspartic Acid............................... 13.31 mg
L-Cysteine Hydrochloride Hydrate.............. 35.12 mg
L-Glutamic Acid............................... 14.71 mg
L-Glutamine................................... 146 mg
Glycine......................................... 7.51 mg
L-Histidine Hydrochloride Hydrate............ 20.96 mg
L-Isoleucine................................... 3.94 mg
L-Leucine...................................... 13.12 mg
L-Lysine Hydrochloride........................ 4.48 mg
L-Phenylalanine............................... 4.96 mg
L-Proline....................................... 34.53 mg
L-Serine........................................ 10.51 mg
L-Threonine..................................... 11.91 mg
L-Tryptophan.................................... 2.04 mg
L-Tyrosine...................................... 5.44 mg
L-Valine........................................ 11.71 mg
D-Biotin........................................ 0.0073 mg
Choline Chloride.............................. 14 mg
Calcium Pantothenate.......................... 0.477 mg
Folic Acid...................................... 1.32 mg
Hypoxanthin................................... 4.08 mg
Myo-Inositol................................... 18 mg
Putrescine Dihydrochloride.................... 0.161 mg
Pyridoxine Hydrochloride...................... 0.062 mg
Riboflavin...................................... 0.038 mg
Sodium Pyruvate............................... 110 mg
Thiamine Hydrochloride........................ 0.377 mg
Thymidine....................................... 0.727 mg
Cyanocobalamin............................... 1.36 mg
Nicotinamide................................... 0.037 mg
α-Lipoic Acid................................... 0.206 mg
Linoleic Acid................................... 0.084 mg
Phenol Red...................................... 1.24 mg
Other substances to be added
Sodium Bicarbonate.......................... As Needed
