产品名称：Recombinant Mouse Osteopontin/OPN Protein

规格或纯度：无动物源, 无载体, 生物活性, ActiBioPure™, 高性能, His标签, ≥95%(SDS-PAGE)

应用：SDS-PAGE, Cell Culture, Functional Studies

生物活性：Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10^5 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 ℃. The ED50 for this effect is 0.25-1.5 µg/mL. Recombinant Human Coagulation Factor II/Thrombin proteolytic treatment of this rmOsteopontin can increase HEK293 cell adhesion by about 10-fold.

氨基酸：17-294 aa (E99G)

无载体：Yes

无动物源：Yes

Accession #：Q547B5

预测分子量：31.5 kDa

SDS-PAGE：30 kDa and 65 kDa under reducing conditions.

级别：无动物源, 无载体, 生物活性, ActiBioPure™, 高性能, His标签

储存缓冲液：Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.

复溶：Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.

储存条件：-20°C储存,避免反复冻融

运输条件：超低温运输

稳定性与储存：在 -20~-80℃ 下可保存 1 年以上。复溶后建议分装。避免冷冻/解冻循环。

Accession #：Q547B5

生物活性：Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10^5 cells/well are added to a Recombinant Mouse (rm) Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 ℃. The ED50 for this effect is 0.25-1.5 µg/mL. Recombinant Human Coagulation Factor II/Thrombin proteolytic treatment of this rmOsteopontin can increase HEK293 cell adhesion by about 10-fold.

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