71206 Millipore
内切核酸酶 来源于粘质沙雷氏菌
Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions
别名：Endonuclease from Serratia marcescens
CAS号：9025-65-4
EC 号：3.1.30.2 (BRENDA, IUBMB)
MDL编号：MFCD00131010
属性
测定
>99% (SDS-PAGE)
形式
liquid
manufacturer/tradename
Novagen®
储存条件
OK to freeze
杂质
<0.25 EU/kU Total endotoxin
运输
wet ice
储存温度
−20°C
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说明
一般描述
Benzonase® Nuclease is a geneticallyengineered endonuclease from Serratiamarcescens. It degrades all forms of DNA andRNA (single stranded, double stranded, linearand circular) while having no proteolyticactivity. It is effective over a wide range ofconditions and possesses an exceptionallyhigh specific activity. The enzyme completelydigests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases inlength (below the hybridization limit), whichis ideal for removal of nucleic acids fromrecombinant proteins, enabling compliancewith FDA guidelines for nucleic acidcontamination. The ability of Benzonase torapidly hydrolyze nucleic acids makes theenzyme an excellent choice for viscosityreduction to reduce processing time andincrease yields of protein. For example, theenzyme is compatible with BugBuster andPopCulture Protein Extraction Reagentsand can therefore be added along with thesereagents to eliminate viscosity and removenucleic acids from E. coli extracts.
The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionicdetergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.
Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 106 U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedas a 0.2 µm filtered solution in 50% glycerol.Store at -20°C.
Total endotoxin:<0.25 EU/1,000 units.Purity: >99% by SDS-PAGE.
包装
25 ku in Glass bottle
应用
用于去除蛋白样品中的核酸。
生化/生理作用
消化天然或热变性的DNA和RNA。
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
法律信息
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
安全信息
储存分类代码
10 - Combustible liquids
WGK
WGK 1
