In Vitro: S1R agonist 1 (Compound 6b; 0.1-5 μM) significantly increases the nerve growth factor (NGF) induced neurite outgrowth at all the tested concentrations in a dose-dependent manner in PC12 cell lines. S1R agonist 1 (24 h) significantly prevents cell damage induced by Rotenone (HY-B1756) when tested at the concentration of 1 μM in SHSY5Y cells. S1R agonist 1 (0.1-5 μM; 24 h) demonstrates a neuroprotective effect against NMDA stimuli in SHSY5Y cells. S1R agonist 1 (0-10 μM; 24-72 h) shows no cytotoxicity against A549, LoVo and Panc-1 cells.
In Vivo: S1R agonist 1 (Compound 6b; 0.1-50 μM; 120 h) induces the death of 4 Zebrafish embryos out of 8 at 10 μM.