In Vitro: Spiroxatrine (0.01-0.1 μM, 15 mins) increases contraction in vas deferenstissues from α2A/D-adrenoceptor knockout mice.
In Vivo: Spiroxatrine (1-25 ug for i.p., 5 days) increases hindpaw withdrawal latencies to thermal and mechanical stimulation in the nerve injury rat and Carrageenan (HY-125474)-induced rat inflammation model. Spiroxatrine (4 mg/kg/day for i.p., 5 mins) increases the voluntary oral ethanol intake induced by Fluoxetine (HY-B0102) in the selectively bred alcohol-preferring P line of rats .