In Vitro: DRP1i27 (0-50 μM) dihydrochloride directly binds to and inhibits GTPase activity of human Drp1. DRP1i27 (0-50 μM) dihydrochloride is able to increase cellular networks of mitochondria in human and mouse fibroblasts in a Drp1-dependent manner. DRP1i27 dihydrochloride has a binding affinity of 286 μM in the SPR assay and a KD value of 190 μM via the MST assay.